Cell Cycle Staining Kit

Quantification of cellular DNA content is a commonly used method for monitoring cell cycle progression. As cells progress through the cell cycle, the amount of DNA ultimately doubles. This doubling can be tracked and used to determine the cell cycle phase (G1, S, and/or G2/M). Cells in the G1 phase have one set of paired chromosomes. During the S phase cellular DNA begins to double, so that the amount of DNA is between one and two times the amount in G1. Cells in G2/M phase have double the amount of DNA compared to cells in G1 and two sets of paired chromosomes. Thus, the amount of DNA in cells directly reflect which cell cycle phase the cells are in.

This kit contains 50 ml of DNA staining solution (containing RNase A) and 500 μl of membrane disruptor, sufficient for 50 detections.