EasyGo!™ Human MMP-9 One-Step ELISA Kit

EK1M09EG

EasyGo!™ Human MMP-9 One-Step ELISA Kit

$349.00 – $499.00

 DATASHEET
Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100uL(diluent)
Sensitivity8.95pg/ml
Array Range125-8000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
SKU: EK1M09EG Category:

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100uL(diluent)
Sensitivity8.95pg/ml
Array Range125-8000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
Intra Precision< 10%
Inter Precision< 10%
PlateDetachable 96-well Plate
Storage2-8℃
Deliveryblue ice
ComponentsCoated Microplate
standard
Assay Buffer (10×)
TMB
Stop Solution
Washing Buffer (20×)
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

MMP9

MMP9 Target Infomation Overview

  • Target Symbol: MMP9, matrix metallopeptidase 9
  • Gene Groups: M10 matrix metallopeptidases
  • Previous Names: CLG4B
  • Alias Names: matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

MMP9, matrix metallopeptidase 9 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human MMP9 Target Information

  • Target Symbol: MMP9, matrix metallopeptidase 9
  • Alias:
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG4B
    • GELB
    • macrophage gelatinase
    • MANDP2
    • matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • matrix metalloproteinase-9
    • MMP-9
    • type V collagenase
  • NCBI_Gene: 4318
  • UniProtKB: P14780

Human MMP9 Predicted Functions

Enables identical protein binding activity; metalloendopeptidase activity; and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of apoptotic signaling pathway; and positive regulation of keratinocyte migration. Located in extracellular space. Implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and chronic obstructive pulmonary disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); brain disease (multiple); carcinoma (multiple); and lung disease (multiple).

Mouse Mmp9 Target Information

Mouse Mmp9 Predicted Functions

Enables metalloendopeptidase activity. Involved in negative regulation of epithelial cell differentiation involved in kidney development and response to amyloid-beta. Acts upstream of or within several processes, including collagen catabolic process; embryo implantation; and extracellular matrix organization. Located in collagen-containing extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; limb mesenchyme; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and lung disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).

Rat Mmp9 Target Information

Rat Mmp9 Predicted Functions

Enables fibronectin binding activity and metallopeptidase activity. Involved in several processes, including cellular response to cell-matrix adhesion; cellular response to cytokine stimulus; and cellular response to iron(III) ion. Located in extracellular space. Part of protein-containing complex. Used to study artery disease (multiple); brain ischemia (multiple); congestive heart failure; glomerulosclerosis; and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); brain disease (multiple); chronic obstructive pulmonary disease (multiple); glucose metabolism disease (multiple); and kidney disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and chronic obstructive pulmonary disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).

Products with the Same Target - MMP9

Rat Total MMP-9 ELISA Kit

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Mouse Total MMP-9 ELISA Kit

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Human MMP-9 ELISA Kit

$299.00 – $419.00
Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

Continue Reading Competitive ELISA: Principles, Methods, and Key Differences

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