Powered by BiozEasyGo!™ Mouse CD5 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 10uL |
| Sensitivity | 3.07 pg/ml |
| Array Range | 12.50-800pg/ml |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
CD5
CD5 Target Infomation Overview
- Target Symbol: CD5, CD5 molecule
- Gene Groups: CD molecules; Scavenger receptor cysteine rich domain containing
- Alias: T1
- Previous Names: LEU1
- Alias Names: CD5 antigen (p56-62)
CD5, CD5 molecule Target Infomation by Species
- Human
- Mouse
- Rat
Human CD5 Target Information
- Target Symbol: CD5, CD5 molecule
- Alias:
- cd5 antigen
- CD5 antigen (p56-62)
- LEU1
- lymphocyte antigen T1/Leu-1
- T-cell surface glycoprotein CD5
- T1
- NCBI_Gene: 921
- UniProtKB: P06127
Human CD5 Predicted Functions
Predicted to enable scavenger receptor activity. Predicted to be involved in T cell costimulation. Predicted to act upstream of or within apoptotic signaling pathway. Located in plasma membrane.
Mouse Cd5 Target Information
- Target Symbol: Cd5, CD5 antigen
- Alias:
- Ly-1
- Ly-12
- Ly-A
- lymphocyte antigen 1
- lymphocyte antigen 12
- Lyt-1
- T-lymphocyte antigen 1
- NCBI_Gene: 12507
Mouse Cd5 Predicted Functions
Predicted to enable scavenger receptor activity. Acts upstream of or within T cell costimulation and apoptotic signaling pathway. Located in external side of plasma membrane. Is expressed in gut and male reproductive gland or organ. Orthologous to human CD5 (CD5 molecule).
Rat Cd5 Target Information
- Target Symbol: Cd5, Cd5 molecule
- Alias:
- CD5 antigen
- LOC100911215
- lymphocyte antigen CD5
- MGC114334
- T-cell surface glycoprotein CD5
- T-cell surface glycoprotein CD5-like
- NCBI_Gene: 54236
Rat Cd5 Predicted Functions
Predicted to enable scavenger receptor activity. Predicted to be involved in T cell costimulation. Predicted to act upstream of or within apoptotic signaling pathway. Is spanning component of plasma membrane. Orthologous to human CD5 (CD5 molecule).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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