Powered by BiozEasyGo!™ Mouse CD55/DAF One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100uL(diluent) |
| Sensitivity | 1.78 pg/ml |
| Array Range | 15.625-1000pg/ml |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
CD55
CD55 Target Infomation Overview
- Target Symbol: CD55, CD55 molecule (Cromer blood group)
- Gene Groups: Blood group antigens; CD molecules; Sushi domain containing; Complement system regulators and receptors
- Alias: CR; TC; CROM
- Previous Names: DAF
- Alias Names: decay accelerating factor for complement (CD55, Cromer blood group system); CD55 molecule, decay accelerating factor for complement (Cromer blood group)
CD55, CD55 molecule (Cromer blood group) Target Infomation by Species
- Human
- Mouse
- Rat
Human CD55 Target Information
- Target Symbol: CD55, CD55 molecule (Cromer blood group)
- Alias:
- CD55 antigen
- CD55 molecule, decay accelerating factor for complement (Cromer blood group)
- CHAPLE
- complement decay-accelerating factor
- CR
- CROM
- Cromer blood group antigen
- DAF
- decay accelerating factor for complement
- Rh blood group D antigen
- TC
- NCBI_Gene: 1604
- UniProtKB: P08174
Human CD55 Predicted Functions
Enables lipid binding activity and virus receptor activity. Involved in several processes, including positive regulation of CD4-positive, alpha-beta T cell proliferation; positive regulation of T cell cytokine production; and regulation of lipopolysaccharide-mediated signaling pathway. Acts upstream of or within regulation of complement activation and regulation of complement-dependent cytotoxicity. Located in cell surface; membrane raft; and plasma membrane. Implicated in prostate cancer and protein-losing enteropathy. Biomarker of breast cancer; cervical cancer; and endometrial adenocarcinoma.
Mouse Cd55 Target Information
- Target Symbol: Cd55, CD55 molecule, decay accelerating factor for complement
- Alias:
- complement-glycosylphosphatidylinositol
- Cromer blood group
- Daf-GPI
- Daf1
- decay accelerating factor 1
- GPI-DAF
- NCBI_Gene: 13136
Mouse Cd55 Predicted Functions
Predicted to enable enzyme inhibitor activity; lipid binding activity; and virus receptor activity. Acts upstream of or within regulation of complement activation, classical pathway. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; liver and biliary system; and nervous system. Human ortholog(s) of this gene implicated in prostate cancer and protein-losing enteropathy. Orthologous to human CD55 (CD55 molecule (Cromer blood group)).
Rat Cd55 Target Information
- Target Symbol: Cd55, CD55 molecule (Cromer blood group)
- Alias:
- CD55 antigen
- CD55 molecule, decay accelerating factor for complement
- complement decay-accelerating factor
- Daf
- Daf1
- decay accelarating factor 1
- decay accelerating factor 1
- decay accelerating factor GPI-form
- decay-accelarating factor
- glycosylphophatidylinositol-anchored form
- NCBI_Gene: 64036
Rat Cd55 Predicted Functions
Enables enzyme inhibitor activity. Involved in several processes, including maternal process involved in parturition; negative regulation of complement activation; and spermatogenesis. Located in apical plasma membrane. Used to study neuroblastoma and proteinuria. Human ortholog(s) of this gene implicated in prostate cancer and protein-losing enteropathy. Orthologous to human CD55 (CD55 molecule (Cromer blood group)).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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