Powered by BiozEasyGo!™ Mouse Factor VII/F7 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100uL(diluent) |
| Sensitivity | 1.77 pg/mL |
| Array Range | 31.25-2000pg/mL |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
F7
F7 Target Infomation Overview
- Target Symbol: F7, coagulation factor VII
- Gene Groups: Gla domain containing
- Alias: eptacog alfa; FVII coagulation protein; factor VII, coagulation factor VII (serum prothrombin conversion accelerator)
F7, coagulation factor VII Target Infomation by Species
- Human
- Mouse
- Rat
Human F7 Target Information
- Target Symbol: F7, coagulation factor VII
- Alias:
- coagulation factor VII (serum prothrombin conversion accelerator)
- eptacog alfa
- FVII coagulation protein
- FVIIa
- proconvertin
- serum prothrombin conversion accelerator
- SPCA
- NCBI_Gene: 2155
- UniProtKB: P08709
Human F7 Predicted Functions
Contributes to serine-type endopeptidase activity. Involved in several processes, including blood coagulation; positive regulation of chemotaxis; and positive regulation of signal transduction. Located in collagen-containing extracellular matrix. Part of serine-type peptidase complex. Implicated in several diseases, including artery disease (multiple); cerebral infarction; diabetes mellitus (multiple); factor VII deficiency; and obesity. Biomarker of several diseases, including angioedema (multiple); artery disease (multiple); diabetic neuropathy; glucose metabolism disease (multiple); and kidney disease (multiple).
Mouse F7 Target Information
- Target Symbol: F7, coagulation factor VII
- Alias:
- AI132620
- Cf7
- expressed sequence AI132620
- FVII
- NCBI_Gene: 14068
Mouse F7 Predicted Functions
Predicted to enable serine-type endopeptidase activity and signaling receptor binding activity. Acts upstream of or within blood coagulation. Predicted to be located in vesicle. Predicted to be part of serine-type peptidase complex. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); cerebral infarction; diabetes mellitus (multiple); factor VII deficiency; and obesity. Orthologous to human F7 (coagulation factor VII).
Rat F7 Target Information
- Target Symbol: F7, coagulation factor VII
- Alias:
- coagulation factor VII (serum prothrombin conversion accelerator)
- serum prothrombin conversion accelerator
- NCBI_Gene: 260320
Rat F7 Predicted Functions
Enables endopeptidase activity and signaling receptor binding activity. Involved in several processes, including response to growth hormone; response to thyrotropin-releasing hormone; and response to thyroxine. Located in extracellular space and vesicle. Used to study asthma; diabetes mellitus; and type 2 diabetes mellitus. Biomarker of several diseases, including bilirubin metabolic disorder; familial hyperlipidemia; hypertension; hypothyroidism; and prothrombin deficiency. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); cerebral infarction; diabetes mellitus (multiple); factor VII deficiency; and obesity. Orthologous to human F7 (coagulation factor VII).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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