EK1410

Human cTnI ELISA Kit

$299.00$419.00

 DATASHEET
Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples
Sample VolumeSerum, plasma: 10 μL, cell culture supernatant: 100 μL
Sensitivity4.67 pg/mL
Array Range125 pg/mL - 8000 pg/mL
Assay Time3.5 h
Recovery90% - 120%
Average Recovery109%
SKU: EK1410 Category:

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples
Sample VolumeSerum, plasma: 10 μL, cell culture supernatant: 100 μL
Sensitivity4.67 pg/mL
Array Range125 pg/mL - 8000 pg/mL
Assay Time3.5 h
Recovery90% - 120%
Average Recovery109%
Intra Precision6.4% - 8.4%
Inter Precision3.5% - 6.6%
PlateDetachable 96-well Plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ Blue Ice Transport
Components1× Washing Buffer
1× Assay Buffer
Stop Solution
Substrate TMB
Detect Antibody
Standard Diluent
Streptavidin-HRP
Standard
Microplate Wells
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human cTnI has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for cTnI are pipetted into the wells and cTnI present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of cTnI bound in the initial step. The color development is stopped and the intensity of the color is measured

Related Targets

TNNI3

TNNI3 Target Infomation Overview

  • Target Symbol: TNNI3, troponin I3, cardiac type
  • Gene Groups: Troponin complex subunits
  • Alias: TNNC1, CMH7, cTNI, CMD2A
  • Alias Names: cardiomyopathy, dilated 2A (autosomal recessive); troponin I type 3 (cardiac); troponin I type 3

TNNI3, troponin I3, cardiac type Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human TNNI3 Target Information

  • Target Symbol: TNNI3, troponin I3, cardiac type
  • Alias:
    • cardiac troponin I
    • cardiomyopathy, dilated 2A (autosomal recessive)
    • CMD1FF
    • CMD2A
    • CMH7
    • cTnI
    • familial hypertrophic cardiomyopathy 7
    • MGC116817
    • RCM1
    • TNNC1
    • troponin 1, type 3
    • troponin I type 3 (cardiac)
    • troponin I, cardiac muscle
  • NCBI_Gene: 7137
  • UniProtKB: P19429

Human Tnni3 Predicted Functions

Enables several functions, including calcium channel inhibitor activity; calcium-dependent protein binding activity; and cytoskeletal protein binding activity. Involved in cardiac muscle contraction; regulation of cardiac muscle contraction by calcium ion signaling; and ventricular cardiac muscle tissue morphogenesis. Located in cardiac myofibril. Part of cardiac Troponin complex. Implicated in intrinsic cardiomyopathy (multiple). Biomarker of coronary artery disease.

Mouse Tnni3 Target Information

Mouse Tnni3 Predicted Functions

Predicted to enable several functions, including calcium channel inhibitor activity; calcium-dependent protein binding activity; and cytoskeletal protein binding activity. Acts upstream of or within several processes, including circulatory system development; intracellular calcium ion homeostasis; and regulation of systemic arterial blood pressure by ischemic conditions. Located in sarcomere. Is expressed in several structures, including alimentary system; central nervous system; heart; integumental system; and sensory organ. Used to study hypertrophic cardiomyopathy 7 and restrictive cardiomyopathy. Human ortholog(s) of this gene implicated in intrinsic cardiomyopathy (multiple). Orthologous to human TNNI3 (troponin I3, cardiac type).

Rat Tnni3 Target Information

  • Target Symbol: Tnni3, troponin I3, cardiac type
  • Alias:
    • cardiac troponin I
    • cTNI
    • TnI
    • troponin 1, type 3
    • Troponin I
    • troponin I cardiac
    • troponin I type 3 (cardiac)
    • troponin I, cardiac
    • troponin I, cardiac muscle
  • NCBI_Gene: 29248
  • UniprotKB: P23693

Rat Tnni3 Predicted Functions

Predicted to enable several functions, including calcium channel inhibitor activity; calcium-dependent protein binding activity; and cytoskeletal protein binding activity. Involved in cardiac muscle contraction and regulation of smooth muscle contraction. Located in sarcomere. Human ortholog(s) of this gene implicated in intrinsic cardiomyopathy (multiple). Orthologous to human TNNI3 (troponin I3, cardiac type).

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