Human IFN-γ High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSIFNG
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity0.04 pg/mL
Range0.39 pg/mL – 25 pg/mL
Assay Time3.5 h
Recovery85% – 94%
Average Recovery0.89
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity0.04 pg/mL
Range0.39 pg/mL – 25 pg/mL
Assay Time3.5 h
Recovery85% – 94%
Average Recovery0.89
Intra Precision4.3% – 5.5%
Inter-Precision4.7% – 7.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IFN-γ monoclonal antibody
Human IFN-γ freeze-dried standard
IFN-γ detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IFN-γ antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IFN-γ present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IFN-γ in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IFNG

IFNG Target Infomation Overview

  • Target Symbol: IFNG, interferon gamma
  • Gene Groups: Interferons

IFNG, interferon gamma Target Infomation by Species

HumanMouseRat

Human IFNG Target Information

Human IFNG Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of cell death; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including positive regulation of gene expression; positive regulation of protein phosphorylation; and response to virus. Located in extracellular region. Implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Biomarker of several diseases, including acute retinal necrosis syndrome; allergic rhinitis; autoimmune disease (multiple); nephritis (multiple); and otitis media (multiple).

Mouse Ifng Target Information

Mouse Ifng Predicted Functions

Enables cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and regulation of generation of precursor metabolites and energy. Acts upstream of or within several processes, including CD8-positive, alpha-beta T cell differentiation involved in immune response; defense response to other organism; and positive regulation of macromolecule metabolic process. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study several diseases, including aplastic anemia; hepatitis; malaria; medulloblastoma; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including allergic rhinitis; ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).

Rat Ifng Target Information

Rat Ifng Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cell population proliferation; positive regulation of NMDA glutamate receptor activity; and positive regulation of macromolecule metabolic process. Located in several cellular components, including extracellular space; neuron projection; and perikaryon. Used to study several diseases, including Chagas disease; allergic conjunctivitis; allergic rhinitis; renal fibrosis; and ureteral obstruction. Biomarker of several diseases, including abdominal aortic aneurysm; autoimmune thyroiditis; pulpitis; tongue squamous cell carcinoma; and visual epilepsy. Human ortholog(s) of this gene implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).

IFNG Target News

    4.4
    The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1
    JOURNAL OF IMMUNOLOGY
    2.8
    Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease
    International Journal of Chronic Obstructive Pulmonary Disease
    11.3
    Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis
    Biomaterials Research
    10.9
    Targeting and cytotoxicity of chimeric antigen receptor T cells grafted with PD1 extramembrane domain
    Experimental Hematology & Oncology
    8.593
    Hypoxia-associated circPRDM4 promotes immune escape via HIF-1α regulation of PD-L1 in hepatocellular carcinoma
    Experimental Hematology & Oncology
    7.259
    Azithromycin attenuates wheezing after pulmonary inflammation through inhibiting histone H3K27me3 hypermethylation mediated by EZH2
    Clinical Epigenetics
    8
    Single-cell transcriptomics reveals variations in monocytes and Tregs between gout flare and remission
    JCI Insight
    2.088
    Effects of potassium channel knockdown on peripheral blood T lymphocytes and NFAT signaling pathway in Xinjiang Kazak patients with hypertension
    CLINICAL AND EXPERIMENTAL HYPERTENSION
    5.578
    Long-Term Immunogenicity and In Vitro Prophylactic Protective Efficacy of M. tuberculosis Fusion Protein DR2 Combined with Liposomal Adjuvant DIMQ as a Boosting Vaccine for BCG
    ACS Infectious Diseases
    7.9
    (–)-Syringaresinol Attenuates Ulcerative Colitis By Improving Intestinal Epithelial Barrier Function And Inhibiting Inflammatory Responses
    PHYTOMEDICINE
    3.5
    Potential salivary and serum biomarkers for burning mouth syndrome and their relationship with anxiety/depression
    Journal of Dental Sciences
    5.6
    Novaferon gene modification promotes NK92 cell anti-tumor activity
    INTERNATIONAL IMMUNOPHARMACOLOGY
    5
    Develop a PD-1-blockade peptide to reinvigorate T-cell activity and inhibit tumor progress
    EUROPEAN JOURNAL OF PHARMACOLOGY
    14.903
    Universal chimeric Fcγ receptor T cells with appropriate affinity for IgG1 antibody exhibit optimal antitumor efficacy
    Acta Pharmaceutica Sinica B
    2.6
    N6-Methyladenosine (m6A) Reader IGF2BP1 Accelerates Gastric Cancer Development and Immune Escape by Targeting PD-L1
    MOLECULAR BIOTECHNOLOGY
    7.2
    N6-methyladenosine reader protein IGF2BP1 suppresses CD8 + T cells-mediated tumor cytotoxicity and apoptosis in colon cancer
    APOPTOSIS
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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