Human IL-17A High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSIL17A
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.20 pg/mL
Range0.78 pg/mL – 50 pg/mL
Assay Time3.5 h
Recovery80% – 95%
Average Recovery0.86
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.20 pg/mL
Range0.78 pg/mL – 50 pg/mL
Assay Time3.5 h
Recovery80% – 95%
Average Recovery0.86
Intra Precision4.3% – 4.6%
Inter-Precision5.9% – 9.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17A monoclonal antibody
Human IL-17A freeze-dried standard
IL-17A detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-17A antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-17A present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-17A in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL17A

IL17A Target Infomation Overview

  • Target Symbol: IL17A, interleukin 17A
  • Gene Groups: Interleukins
  • Alias: IL-17A; IL-17
  • Previous Names: CTLA8; IL17
  • Alias Names: cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

IL17A, interleukin 17A Target Infomation by Species

HumanMouseRat

Human IL17A Target Information

  • Target Symbol: IL17A, interleukin 17A
  • Alias:
    • CTLA-8
    • CTLA8
    • cytotoxic T-lymphocyte-associated antigen 8
    • cytotoxic T-lymphocyte-associated protein 8
    • cytotoxic T-lymphocyte-associated serine esterase 8
    • IL-17
    • IL-17A
    • IL17
    • ILA17
    • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
    • interleukin-17
    • interleukin-17A
  • NCBI_Gene: 3605
  • UniProtKB: Q16552

Human IL17A Predicted Functions

Predicted to enable cytokine activity; protein heterodimerization activity; and protein homodimerization activity. Involved in several processes, including interleukin-17-mediated signaling pathway; positive regulation of cytokine production; and positive regulation of osteoclast differentiation. Acts upstream of or within positive regulation of cytokine production and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular region. Implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); macular degeneration; and rhinitis (multiple). Biomarker of several diseases, including acne; autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and rhinitis (multiple).

Mouse Il17a Target Information

Mouse Il17a Predicted Functions

Enables protein heterodimerization activity and protein homodimerization activity. Involved in several processes, including defense response to other organism; intestinal epithelial structure maintenance; and positive regulation of gene expression. Acts upstream of or within cellular response to interleukin-1; positive regulation of cytokine production involved in inflammatory response; and positive regulation of transcription by RNA polymerase II. Located in external side of plasma membrane and extracellular space. Human ortholog(s) of this gene implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); eye disease (multiple); and rhinitis (multiple). Orthologous to human IL17A (interleukin 17A).

Rat Il17a Target Information

  • Target Symbol: Il17a, interleukin 17A
  • Alias:
    • CTLA-8
    • cytotoxic T-lymphocyte-associated antigen 8
    • hypothetical protein LOC301289
    • IL-17
    • IL-17A
    • Il17
    • interleukin 17
    • Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
    • interleukin-17A
    • LOC301289
    • similar to Interleukin-17 precursor (IL-17) (Cytotoxic T lymphocyte-associated antigen 8) (CTLA-8)
  • NCBI_Gene: 301289

Rat Il17a Predicted Functions

Predicted to enable protein heterodimerization activity and protein homodimerization activity. Involved in several processes, including cellular response to glucocorticoid stimulus; positive regulation of necrotic cell death; and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and extracellular space. Used to study anti-basement membrane glomerulonephritis; congestive heart failure; myocarditis; pleurisy; and uveitis. Biomarker of several diseases, including acute necrotizing pancreatitis; inflammatory bowel disease (multiple); lipoid nephrosis; lung disease (multiple); and periodontal disease (multiple). Human ortholog(s) of this gene implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); macular degeneration; and rhinitis (multiple). Orthologous to human IL17A (interleukin 17A).

IL17A Target News

    2.8
    Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease
    International Journal of Chronic Obstructive Pulmonary Disease
    11.3
    Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis
    Biomaterials Research
    3.777
    Hyperoside alleviates postmenopausal osteoporosis via regulating miR-19a-5p/IL-17A axis
    AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
    5.195
    Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro
    JOURNAL OF ETHNOPHARMACOLOGY
    3.7
    A role of NR4A2 in Graves’ disease: regulation of Th17/Treg
    ENDOCRINE
    5.8
    BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
    3.124
    MicroRNA-143-3p ameliorates collagen-induced arthritis by polarizing naive CD4+T cells into Treg cells
    JOURNAL OF CLINICAL LABORATORY ANALYSIS
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus

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