Human Vitronectin/VTN ELISA Kit

$350.00$450.00

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TARGETSVTN
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity13.41 pg/mL
Range0.25 ng/mL – 16 ng/mL
Assay Time3.5 h
Recovery95% – 105%
Average Recovery0.98
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity13.41 pg/mL
Range0.25 ng/mL – 16 ng/mL
Assay Time3.5 h
Recovery95% – 105%
Average Recovery0.98
Intra Precision3.9% – 6.7%
Inter-Precision2.6% – 4.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Vitronectin/VTN monoclonal antibody
Human Vitronectin/VTN freeze-dried standard
Vitronectin/VTN detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human VTN antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, VTN present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of VTN in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

VTN

VTN Target Infomation Overview

  • Target Symbol: VTN, vitronectin
  • Gene Groups: Receptor ligands; Complement system regulators and receptors
  • Alias: VN
  • Alias Names: serum spreading factor; somatomedin B; complement S-protein; vitronectin (serum spreading factor, somatomedin B, complement S-protein)

VTN, vitronectin Target Infomation by Species

HumanMouseRat

Human VTN Target Information

  • Target Symbol: VTN, vitronectin
  • Alias:
    • complement S-protein
    • epibolin
    • S-protein
    • serum spreading factor
    • serum-spreading factor
    • somatomedin B
    • V75
    • vitronectin (serum spreading factor, somatomedin B, complement S-protein)
    • VN
    • VNT
  • NCBI_Gene: 7448
  • UniProtKB: P04004

Human VTN Predicted Functions

Enables integrin binding activity. Involved in several processes, including endodermal cell differentiation; regulation of cellular protein metabolic process; and smooth muscle cell-matrix adhesion. Located in collagen-containing extracellular matrix; endoplasmic reticulum; and extracellular space. Implicated in Churg-Strauss syndrome; granulomatosis with polyangiitis; and thrombosis. Biomarker of coronary artery disease and diabetic retinopathy.

Mouse Vtn Target Information

Mouse Vtn Predicted Functions

Enables extracellular matrix binding activity. Involved in oligodendrocyte differentiation. Acts upstream of or within cell-matrix adhesion; extracellular matrix organization; and positive regulation of cell-substrate adhesion. Located in extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; embryo mesenchyme; metanephros; and retina. Human ortholog(s) of this gene implicated in Churg-Strauss syndrome; granulomatosis with polyangiitis; and thrombosis. Orthologous to human VTN (vitronectin).

Rat Vtn Target Information

Rat Vtn Predicted Functions

Enables collagen binding activity; heparin binding activity; and identical protein binding activity. Involved in liver regeneration and protein polymerization. Located in several cellular components, including Golgi lumen; basement membrane; and rough endoplasmic reticulum lumen. Used to study proteinuria. Biomarker of diabetic retinopathy and visual epilepsy. Human ortholog(s) of this gene implicated in Churg-Strauss syndrome; granulomatosis with polyangiitis; and thrombosis. Orthologous to human VTN (vitronectin).

VTN Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis

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