Rat IFN-γ ELISA Kit

$350.00$450.00

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TARGETSIFNG
SPECIESRat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.54 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery87% – 112%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.54 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery87% – 112%
Average Recovery0.99
Intra Precision4.1% – 6.0%
Inter-Precision5.4% – 6.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IFN-γ monoclonal antibody
Rat IFN-γ freeze-dried standard
IFN-γ detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IFN-γ antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IFN-γ present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IFN-γ in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IFNG

IFNG Target Infomation Overview

  • Target Symbol: IFNG, interferon gamma
  • Gene Groups: Interferons

IFNG, interferon gamma Target Infomation by Species

HumanMouseRat

Human IFNG Target Information

Human IFNG Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of cell death; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including positive regulation of gene expression; positive regulation of protein phosphorylation; and response to virus. Located in extracellular region. Implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Biomarker of several diseases, including acute retinal necrosis syndrome; allergic rhinitis; autoimmune disease (multiple); nephritis (multiple); and otitis media (multiple).

Mouse Ifng Target Information

Mouse Ifng Predicted Functions

Enables cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and regulation of generation of precursor metabolites and energy. Acts upstream of or within several processes, including CD8-positive, alpha-beta T cell differentiation involved in immune response; defense response to other organism; and positive regulation of macromolecule metabolic process. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study several diseases, including aplastic anemia; hepatitis; malaria; medulloblastoma; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including allergic rhinitis; ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).

Rat Ifng Target Information

Rat Ifng Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cell population proliferation; positive regulation of NMDA glutamate receptor activity; and positive regulation of macromolecule metabolic process. Located in several cellular components, including extracellular space; neuron projection; and perikaryon. Used to study several diseases, including Chagas disease; allergic conjunctivitis; allergic rhinitis; renal fibrosis; and ureteral obstruction. Biomarker of several diseases, including abdominal aortic aneurysm; autoimmune thyroiditis; pulpitis; tongue squamous cell carcinoma; and visual epilepsy. Human ortholog(s) of this gene implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).

IFNG Target News

    6.2
    Identification of Clec7a as the therapeutic target of rTMS in alleviating Parkinson’s disease: targeting neuroinflammation
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
    6.058
    M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats
    Neural Regeneration Research
    9.8
    Chronic cold environment regulates rheumatoid arthritis through modulation of gut microbiota-derived bile acids
    SCIENCE OF THE TOTAL ENVIRONMENT
    7.9
    Wnt/β-catenin signaling pathway promotes abnormal activation of fibroblast-like synoviocytes and angiogenesis in rheumatoid arthritis and the intervention of Er Miao San
    PHYTOMEDICINE
    8.4
    Microneedle-mediated transdermal delivery of FK506 for treatment of acute cardiac allograft rejection
    MATERIALS & DESIGN
    7.5
    Paeoniflorin inhibits hepatocellular carcinoma growth by reducing PD-L1 expression
    BIOMEDICINE & PHARMACOTHERAPY
    6.2
    Identification of Clec7a as the therapeutic target of rTMS in alleviating Parkinson’s disease: targeting neuroinflammation
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
    14.5
    An injectable signal-amplifying device elicits a specific immune response against malignant glioblastoma
    Acta Pharmaceutica Sinica B
    6.575
    Integrating widely targeted lipidomics and transcriptomics unravels aberrant lipid metabolism and identifies potential biomarkers of food allergies in rats
    MOLECULAR NUTRITION & FOOD RESEARCH
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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