Rat IL-1β High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSIL1B
SPECIESRat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.71 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery89% – 127%
Average Recovery1.06
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.71 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery89% – 127%
Average Recovery1.06
Intra Precision3.8% – 5.2%
Inter-Precision4.9% – 5.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1β monoclonal antibody
Rat IL-1β freeze-dried standard
IL-1β detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-1β antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-1β present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL1B

IL1B Target Infomation Overview

  • Target Symbol: IL1B, interleukin 1 beta
  • Gene Groups: Interleukins
  • Alias: IL1F2; IL-1B; IL1-BETA

IL1B, interleukin 1 beta Target Infomation by Species

HumanMouseRat

Human IL1B Target Information

  • Target Symbol: IL1B, interleukin 1 beta
  • Alias:
    • catabolin
    • IL-1
    • IL-1 beta
    • IL1-BETA
    • IL1beta
    • IL1F2
    • interleukin 1, beta
    • interleukin 1beta
    • interleukin-1 beta
    • preinterleukin 1 beta
    • pro-interleukin-1-beta
  • NCBI_Gene: 3553
  • UniProtKB: P01584

Human IL1B Predicted Functions

Enables cytokine activity; integrin binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of nitrogen compound metabolic process; and regulation of signal transduction. Acts upstream of or within cellular response to organic substance and cellular response to xenobiotic stimulus. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bronchial disease (multiple); lung disease (multiple); and nose disease (multiple).

Mouse Il1b Target Information

Mouse Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

Rat Il1b Target Information

Rat Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

IL1B Target News

    1.49
    6-Gingerol attenuates sepsis-induced acute lung injury by suppressing NLRP3 inflammasome through Nrf2 activation
    FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
    1.49
    HDAC6 inhibition alleviates acute pulmonary embolism: a possible future therapeutic option
    FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
    4.927
    Inhibition of TLR4 Alleviates Heat Stroke-Induced Cardiomyocyte Injury by Down-Regulating Inflammation and Ferroptosis
    MOLECULES
    3.115
    Gastrodin Prevents Neuronal Apoptosis and Improves Neurological Deficits in Traumatic Brain Injury Rats through PKA/CREB/Bcl2 Axis
    Frontiers in Bioscience-Landmark
    5.2
    Astaxanthin prevents osteoarthritis by blocking Rspo2-mediated Wnt/β-catenin signaling in chondrocytes and abolishing Rspo2-related inflammatory factors in macrophages
    Aging-US
    1.798
    FERMT1 knockdown attenuates inflammation induced by intracerebral hemorrhage in rats via NLRP3/NF-κB pathway
    EXPERIMENTAL ANIMALS
    1.798
    SOX9 modulates Müller cell gliosis in diabetic retinopathy through upregulating TXNIP transcription
    EXPERIMENTAL ANIMALS
    2.123
    Lycopene ameliorates locomotor activity and urinary frequency induced by pelvic venous congestion in rats
    Open Medicine
    1.798
    Knockdown of HDAC9 attenuates sepsis-induced myocardial injury and inflammatory response
    EXPERIMENTAL ANIMALS
    1.311
    Coptisine mitigates diabetic nephropathy via repressing the NRLP3 inflammasome
    Open Life Sciences
    8.079
    Mitochondrial transfer from bone mesenchymal stem cells protects against tendinopathy both in vitro and in vivo
    Stem Cell Research & Therapy
    4.9
    Yangqing Chenfei formula alleviates silica-induced pulmonary inflammation in rats by inhibiting macrophage M1 polarization
    Chinese Medicine
    2.838
    Protective effecs of baicalin magnesium on non-alcoholic steatohepatitis rats are based on inhibiting NLRP3/Caspase-1/IL-1β signaling pathway
    BMC Complementary Medicine and Therapies
    3.247
    Hyperoside exerts protective effects against anticardiolipin antibody-induced recurrent pregnancy loss in vivo and in vitro
    HUMAN & EXPERIMENTAL TOXICOLOGY
    4
    Protective Effects of Aqueous Extracts of the Herb of Paederia scandens (Lour.) Merr. against HCl/EtOH-Induced Gastric Ulcer in Rats: Involvement and Inhibitors’ Identification of NF-κB Signaling
    JOURNAL OF FOOD BIOCHEMISTRY
    7.31
    P2X7R Mediates the Synergistic Effect of ATP and MSU Crystals to Induce Acute Gouty Arthritis
    Oxidative Medicine and Cellular Longevity
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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