Rat TNF-α High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSTNF
SPECIESRat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.73 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery92% – 127%
Average Recovery1.12
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.73 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery92% – 127%
Average Recovery1.12
Intra Precision4.2% – 4.8%
Inter-Precision3.7% – 10.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody
Rat TNF-α freeze-dried standard
TNF-α detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat TNF-α antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the TNF-α present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TNF-α in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TNF

TNF Target Infomation Overview

  • Target Symbol: TNF, tumor necrosis factor
  • Gene Groups: Tumor necrosis factor superfamily
  • Alias: TNFSF2; DIF; TNF-alpha
  • Previous Names: TNFA
  • Alias Names: TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF, tumor necrosis factor Target Infomation by Species

HumanMouseRat

Human TNF Target Information

  • Target Symbol: TNF, tumor necrosis factor
  • Alias:
    • APC1 protein
    • cachectin
    • DIF
    • TNF superfamily, member 2
    • TNF-a
    • TNF-alpha
    • TNF, macrophage-derived
    • TNF, monocyte-derived
    • TNFA
    • TNFSF2
    • TNLG1F
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand 1F
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor-alpha
  • NCBI_Gene: 7124
  • UniProtKB: P01375

Human TNF Predicted Functions

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

Mouse Tnf Target Information

Mouse Tnf Predicted Functions

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

Rat Tnf Target Information

  • Target Symbol: Tnf, tumor necrosis factor
  • Alias:
    • cachectin
    • LOC103694380
    • MGC124630
    • RATTNF
    • TNF-a
    • TNF-alpha
    • Tnfa
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor superfamily member 2
    • tumor necrosis factor superfamily, member 2
    • tumor necrosis factor-alpha
    • tumor necrosis factor-like
  • NCBI_Gene: 24835
  • UniProtKB: P16599

Rat Tnf Predicted Functions

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

TNF Target News

    1.49
    HDAC6 inhibition alleviates acute pulmonary embolism: a possible future therapeutic option
    FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
    1.49
    6-Gingerol attenuates sepsis-induced acute lung injury by suppressing NLRP3 inflammasome through Nrf2 activation
    FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
    4.927
    Inhibition of TLR4 Alleviates Heat Stroke-Induced Cardiomyocyte Injury by Down-Regulating Inflammation and Ferroptosis
    MOLECULES
    3.1
    Dexmedetomidine Alleviates Ischemia/Reperfusion-Associated Acute Kidney Injury by Enhancing Autophagic Activity via the α2-AR/AMPK/mTOR Pathway
    Frontiers in Bioscience-Landmark
    3.115
    Gastrodin Prevents Neuronal Apoptosis and Improves Neurological Deficits in Traumatic Brain Injury Rats through PKA/CREB/Bcl2 Axis
    Frontiers in Bioscience-Landmark
    5.2
    Astaxanthin prevents osteoarthritis by blocking Rspo2-mediated Wnt/β-catenin signaling in chondrocytes and abolishing Rspo2-related inflammatory factors in macrophages
    Aging-US
    1.798
    Knockdown of HDAC9 attenuates sepsis-induced myocardial injury and inflammatory response
    EXPERIMENTAL ANIMALS
    1.798
    SIRT3 confers protection against acute pulmonary embolism by anti-inflammation, anti-oxidative stress, anti-apoptosis: participation of AMPK/mTOR pathway
    EXPERIMENTAL ANIMALS
    1.798
    SOX9 modulates Müller cell gliosis in diabetic retinopathy through upregulating TXNIP transcription
    EXPERIMENTAL ANIMALS
    4
    Protective Effects of Aqueous Extracts of the Herb of Paederia scandens (Lour.) Merr. against HCl/EtOH-Induced Gastric Ulcer in Rats: Involvement and Inhibitors’ Identification of NF-κB Signaling
    JOURNAL OF FOOD BIOCHEMISTRY
    6.005
    Baicalin Ameliorates Lung Injury in Rats by Inhibiting NLRP3 Inflammasome Activation via NF-κB Signaling Pathway
    AMERICAN JOURNAL OF CHINESE MEDICINE
    4.8
    GRP78 improves the therapeutic effect of mesenchymal stem cells on hemorrhagic shock-induced liver injury: Involvement of the NF–кB and HO-1/Nrf-2 pathways
    FASEB JOURNAL
    4.8
    Neutrophil extracellular traps facilitate sympathetic hyperactivity by polarizing microglia toward M1 phenotype after traumatic brain injury
    FASEB JOURNAL
    4.1
    TNF-α preconditioned UCMSCs-derived EVs enhance the inhibition of necroptosis of acinar cells in SAP
    Tissue Engineering Part A
    3.222
    Ischemic postconditioning protects against acute kidney injury after limb ischemia reperfusion by regulating HMGB1 release and autophagy
    RENAL FAILURE
    Treatment of Idiopathic Pulmonary Fibrosis by Inhaled Silybin Dry Powder Prepared via the Nanosuspension Spray Drying Technology
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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