Powered by BiozEasyGo!™ Mouse CD8a One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20uL |
| Sensitivity | 7.34 pg/mL |
| Array Range | 46.88-3000pg/mL |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
CD8A
CD8A Target Infomation Overview
- Target Symbol: CD8A, CD8a molecule
- Gene Groups: CD molecules; V-set domain containing
- Previous Names: CD8
- Alias Names: CD8 antigen, alpha polypeptide (p32); T-cell surface glycoprotein CD8 alpha chain
CD8A, CD8a molecule Target Infomation by Species
- Human
- Mouse
- Rat
Human CD8A Target Information
- Target Symbol: CD8A, CD8a molecule
- Alias:
- CD8
- cd8 antigen, alpha chain
- CD8 antigen, alpha polypeptide (p32)
- Cluster of Differentiation 8a
- Leu2
- Leu2 T-lymphocyte antigen
- MAL
- OKT8 T-cell antigen
- p32
- T cell co-receptor
- T-cell antigen Leu2
- T-cell surface glycoprotein CD8 alpha chain
- T-lymphocyte differentiation antigen T8/Leu-2
- T8 T-cell antigen
- NCBI_Gene: 925
- UniProtKB: P01732
Human CD8A Predicted Functions
Enables MHC class I protein complex binding activity. Predicted to be involved in T cell mediated immunity; cell surface receptor signaling pathway; and cytotoxic T cell differentiation. Predicted to act upstream of or within defense response to virus and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane and plasma membrane raft. Biomarker of COVID-19; erythema multiforme; and stomach cancer.
Mouse Cd8a Target Information
- Target Symbol: Cd8a, CD8 antigen, alpha chain
- Alias:
- BB154331
- expressed sequence BB154331
- Ly-2
- Ly-35
- Ly-B
- lymphocyte antigen 2
- lymphocyte antigen 35
- Lyt-2
- T-lymphocyte antigen 2
- NCBI_Gene: 12525
Mouse Cd8a Predicted Functions
Enables identical protein binding activity. Acts upstream of or within several processes, including cytotoxic T cell differentiation; defense response to virus; and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane. Is expressed in several structures, including endocrine gland; exocrine gland; genitourinary system; gut; and hemolymphoid system gland. Orthologous to human CD8A (CD8a molecule).
Rat Cd8a Target Information
- Target Symbol: Cd8a, CD8a molecule
- Alias:
- CD8 antigen 32 kDa chain
- CD8 antigen alpha-chain
- CD8 antigen, alpha chain
- CD8 antigen, alpha-chain
- OX-8 membrane antigen
- T-cell surface glycoprotein CD8 alpha chain
- NCBI_Gene: 24930
- UniProtKB: P07725
Rat Cd8a Predicted Functions
Enables protein kinase binding activity. Involved in T cell activation. Predicted to be located in cell surface and plasma membrane raft. Predicted to be active in external side of plasma membrane. Orthologous to human CD8A (CD8a molecule).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
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