Powered by BiozEasyGo!™ Mouse FGFR3/CD333 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20uL |
| Sensitivity | 55.72 pg/mL |
| Array Range | 156.25-10000pg/mL |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
FGFR3
FGFR3 Target Infomation Overview
- Target Symbol: FGFR3, fibroblast growth factor receptor 3
- Gene Groups: I-set domain containing; Receptor tyrosine kinases; CD molecules
- Alias: CEK2; JTK4; CD333, ACH
- Alias Names: achondroplasia, thanatophoric dwarfism
FGFR3, fibroblast growth factor receptor 3 Target Infomation by Species
- Human
- Mouse
- Rat
Human FGFR3 Target Information
- Target Symbol: FGFR3, fibroblast growth factor receptor 3
- Alias:
- ACH
- achondroplasia, thanatophoric dwarfism
- CD333
- CEK2
- FGFR-3
- fibroblast growth factor receptor 3 variant 4
- fibroblast growth factor receptor 3-S
- HSFGFR3EX
- hydroxyaryl-protein kinase
- JTK4
- tyrosine kinase JTK4
- NCBI_Gene: 2261
- UniProtKB: P22607
Human FGFR3 Predicted Functions
Enables fibroblast growth factor binding activity; fibroblast growth factor receptor activity; and identical protein binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of intracellular signal transduction; and positive regulation of tyrosine phosphorylation of STAT protein. Located in several cellular components, including Golgi apparatus; endoplasmic reticulum; and transport vesicle. Implicated in several diseases, including Human papillomavirus infectious disease; SADDAN; acanthosis nigricans; bone disease (multiple); and carcinoma (multiple). Biomarker of several diseases, including Merkel cell carcinoma; chronic myeloid leukemia; gastrointestinal system cancer (multiple); thanatophoric dysplasia; and urinary bladder cancer (multiple).
Mouse Fgfr3 Target Information
- Target Symbol: Fgfr3, fibroblast growth factor receptor 3
- Alias:
- Fgfr-3
- HBGFR
- sam3
- NCBI_Gene: 14184
Mouse Fgfr3 Predicted Functions
Enables fibroblast growth factor receptor activity and miRNA binding activity. Involved in several processes, including bone development; post-anal tail morphogenesis; and response to sodium phosphate. Acts upstream of or within several processes, including epithelial cell differentiation; negative regulation of cell differentiation; and nervous system development. Located in cytoplasmic side of plasma membrane; lysosome; and perinuclear region of cytoplasm. Is expressed in several structures, including alimentary system; brain; respiratory system; sensory organ; and skeleton. Used to study Muenke Syndrome; achondroplasia; hypochondroplasia; scoliosis; and thanatophoric dysplasia. Human ortholog(s) of this gene implicated in several diseases, including Human papillomavirus infectious disease; SADDAN; acanthosis nigricans; bone disease (multiple); and carcinoma (multiple). Orthologous to human FGFR3 (fibroblast growth factor receptor 3).
Rat Fgfr3 Target Information
- Target Symbol: Fgfr3, fibroblast growth factor receptor 3
- Alias:
- -
- NCBI_Gene: 84489
Rat Fgfr3 Predicted Functions
Enables fibroblast growth factor binding activity and fibroblast growth factor receptor activity. Involved in cell-cell signaling; fibroblast growth factor receptor signaling pathway; and positive regulation of cell population proliferation. Located in cell surface. Biomarker of chondrosarcoma. Human ortholog(s) of this gene implicated in several diseases, including Human papillomavirus infectious disease; SADDAN; acanthosis nigricans; bone disease (multiple); and carcinoma (multiple). Orthologous to human FGFR3 (fibroblast growth factor receptor 3).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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