Powered by BiozEasyGo!™ Mouse IL18R1 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20uL |
| Sensitivity | 0.53 pg/ml |
| Array Range | 6.25-400pg/ml |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
IL18R1
IL18R1 Target Infomation Overview
- Target Symbol: IL18R1, interleukin 18 receptor 1
- Gene Groups: Interleukin receptors; TIR domain containing; CD molecules; Immunoglobulin like domain containing
- Alias: IL1RRP; IL-1Rrp; CD218a
IL18R1, interleukin 18 receptor 1 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL18R1 Target Information
- Target Symbol: IL18R1, interleukin 18 receptor 1
- Alias:
- CD218 antigen-like family member A
- CD218a
- CDw218a
- cytokine receptor
- IL-18R-1
- IL-18R-alpha
- IL-18R1
- IL-18Ralpha
- IL-1Rrp
- IL1 receptor-related protein
- IL18RA
- IL18Ralpha2
- IL1R-rp
- IL1RRP
- interleukin-18 receptor 1
- interleukin-18 receptor alpha
- NCBI_Gene: 8809
- UniProtKB: Q13478
Human IL18R1 Predicted Functions
Enables interleukin-18 binding activity and interleukin-18 receptor activity. Involved in interleukin-18-mediated signaling pathway and regulation of gene expression. Part of interleukin-18 receptor complex. Implicated in asthma. Biomarker of hepatocellular carcinoma; lung disease; pulmonary fibrosis; and pulmonary sarcoidosis.
Mouse Il18r1 Target Information
- Target Symbol: Il18r1, interleukin 18 receptor 1
- Alias:
- Il18ralpha
- Il1rrp
- interleukin 1 receptor related protein
- NCBI_Gene: 16182
Mouse Il18r1 Predicted Functions
Enables interleukin-18 receptor activity. Involved in negative regulation of cold-induced thermogenesis and positive regulation of non-canonical NF-kappaB signal transduction. Acts upstream of or within several processes, including T-helper 1 cell differentiation; interleukin-18-mediated signaling pathway; and positive regulation of type II interferon production. Predicted to be part of interleukin-18 receptor complex. Predicted to be active in cell surface and plasma membrane. Is expressed in aorta-gonad-mesonephros and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL18R1 (interleukin 18 receptor 1).
Rat Il18r1 Target Information
- Target Symbol: Il18r1, interleukin 18 receptor 1
- Alias:
- IL18Ralpha2
- interleukin-18 receptor 1
- LOC301365
- NCBI_Gene: 301365
Rat Il18r1 Predicted Functions
Predicted to enable interleukin-18 binding activity and interleukin-18 receptor activity. Predicted to be involved in several processes, including interleukin-18-mediated signaling pathway; negative regulation of cold-induced thermogenesis; and regulation of gene expression. Predicted to act upstream of or within T-helper 1 cell differentiation and natural killer cell activation. Predicted to be part of interleukin-18 receptor complex. Predicted to be active in cell surface and plasma membrane. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL18R1 (interleukin 18 receptor 1).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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