Human Apolipoprotein AII/Apo A2 ELISA Kit

$350.00$450.00

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TARGETSAPOA2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity0.53 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery91% – 123%
Average Recovery1.06
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity0.53 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery91% – 123%
Average Recovery1.06
Intra Precision3.8% – 4.5%
Inter-Precision4.3% – 9.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein AII/Apo A2 monoclonal antibody
Human Apolipoprotein AII/Apo A2 freeze-dried standard
Apolipoprotein AII/Apo A2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo A2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo A2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo A2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

APOA2

APOA2 Target Infomation Overview

  • Target Symbol: APOA2, apolipoprotein A2
  • Gene Groups: Apolipoproteins
  • Alias Names: apolipoprotein A-II

APOA2, apolipoprotein A2 Target Infomation by Species

HumanMouseRat

Human APOA2 Target Information

Human APOA2 Predicted Functions

Enables several functions, including cholesterol binding activity; protein dimerization activity; and signaling receptor binding activity. Contributes to cholesterol transfer activity. Involved in several processes, including lipid transport; plasma lipoprotein particle organization; and regulation of lipid metabolic process. Located in blood microparticle and extracellular exosome. Part of chylomicron; spherical high-density lipoprotein particle; and very-low-density lipoprotein particle. Implicated in familial hypercholesterolemia. Biomarker of coronary artery disease; familial combined hyperlipidemia; obesity; type 1 diabetes mellitus; and type 2 diabetes mellitus.

Mouse Apoa2 Target Information

Mouse Apoa2 Predicted Functions

Enables high-density lipoprotein particle binding activity. Acts upstream of or within several processes, including cholesterol homeostasis; negative regulation of lipase activity; and regulation of intestinal cholesterol absorption. Located in extracellular space. Is expressed in alimentary system; embryo; liver; liver lobe; and lung. Human ortholog(s) of this gene implicated in familial hypercholesterolemia. Orthologous to human APOA2 (apolipoprotein A2).

Rat Apoa2 Target Information

Rat Apoa2 Predicted Functions

Enables cholesterol transfer activity. Involved in several processes, including animal organ regeneration; response to estrogen; and response to glucocorticoid. Predicted to be located in extracellular space. Predicted to be part of chylomicron; spherical high-density lipoprotein particle; and very-low-density lipoprotein particle. Biomarker of hyperthyroidism. Human ortholog(s) of this gene implicated in familial hypercholesterolemia. Orthologous to human APOA2 (apolipoprotein A2).

APOA2 Target News

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