Human CCL20/MIP-3α ELISA Kit

$350.00$450.00

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TARGETSCCL20
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity8.55 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery87% – 119%
Average Recovery1.01
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity8.55 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery87% – 119%
Average Recovery1.01
Intra Precision3.8% – 4.5%
Inter-Precision3.2% – 5.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL20/MIP-3α monoclonal antibody
Human CCL20/MIP-3α freeze-dried standard
CCL20/MIP-3α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CCL20 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the CCL20 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL20 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL20

CCL20 Target Infomation Overview

  • Target Symbol: CCL20, C-C motif chemokine ligand 20
  • Gene Groups: Chemokine ligands
  • Alias: LARC; MIP-3a; exodus-1; ST38; CKb4
  • Previous Names: SCYA20
  • Alias Names: small inducible cytokine subfamily A (Cys-Cys), member 20; chemokine (C-C motif) ligand 20

CCL20, C-C motif chemokine ligand 20 Target Infomation by Species

HumanMouseRat

Human CCL20 Target Information

  • Target Symbol: CCL20, C-C motif chemokine ligand 20
  • Alias:
    • beta chemokine exodus-1
    • beta-chemokine exodus-1
    • C-C motif chemokine 20
    • CC chemokine LARC
    • chemokine (C-C motif) ligand 20
    • CKb4
    • Exodus
    • exodus-1
    • LARC
    • liver and activation-regulated chemokine
    • macrophage inflammatory protein 3 alpha
    • MIP-3-alpha
    • MIP-3a
    • MIP3A
    • SCYA20
    • small inducible cytokine subfamily A (Cys-Cys), member 20
    • small-inducible cytokine A20
    • ST38
  • NCBI_Gene: 6364
  • UniProtKB: P78556

Human CCL20 Predicted Functions

Enables CCR6 chemokine receptor binding activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; calcium-mediated signaling using intracellular calcium source; and cell chemotaxis. Acts upstream of or within positive regulation of T cell migration. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including IgA glomerulonephritis; Lyme disease; anogenital venereal wart; dermatitis (multiple); and skin papilloma.

Mouse Ccl20 Target Information

Mouse Ccl20 Predicted Functions

Enables CCR6 chemokine receptor binding activity and cytokine activity. Involved in chemotaxis and thymocyte migration. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Orthologous to human CCL20 (C-C motif chemokine ligand 20).

Rat Ccl20 Target Information

  • Target Symbol: Ccl20, C-C motif chemokine ligand 20
  • Alias:
    • beta chemokine exodus-1
    • beta-chemokine exodus-1
    • C-C motif chemokine 20
    • CC chemokine LARC
    • CC chemokine ST38
    • chemokine (C-C motif) ligand 20
    • liver and activation-regulated chemokine
    • macrophage inflammatory protein 3 alpha
    • MIP-3-alpha
    • Scya20
    • small inducible cytokine subfamily A20
    • small-inducible cytokine A20
    • ST38
  • NCBI_Gene: 29538
  • UniProtKB: P97884

Rat Ccl20 Predicted Functions

Enables chemokine activity. Involved in several processes, including cellular response to molecule of bacterial origin; positive regulation of macromolecule metabolic process; and wound healing. Located in extracellular space. Used to study transient cerebral ischemia. Biomarker of brain ischemia and ulcerative colitis. Orthologous to human CCL20 (C-C motif chemokine ligand 20).

CCL20 Target News

    2.3
    Association Between Serum Chemokine Ligand 20 Levels and Disease Activity and Th1/Th2/Th17-Related Cytokine Levels in Rheumatoid Arthritis
    JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis

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