Human CCL26/Eotaxin-3 ELISA Kit

🔍
EK1150

Human CCL26/Eotaxin-3 ELISA Kit

$245.00 – $350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.44 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery81% – 120%
Average Recovery94%
-+
SKU: EK1150 Category:

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.44 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery81% – 120%
Average Recovery94%
Intra Precision2.2% – 4.0%
Inter Precision2.6% – 4.0%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Eotaxin-3 monoclonal antibody
Human Eotaxin-3 freeze-dried standard
Eotaxin-3 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Eotaxin-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Eotaxin-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of Eotaxin-3 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CCL26

CCL26 Target Infomation Overview

  • Target Symbol: CCL26, C-C motif chemokine ligand 26
  • Gene Groups: Chemokine ligands
  • Alias: MIP-4alpha; eotaxin-3; IMAC; MIP-4a; TSC-1
  • Previous Names: SCYA26
  • Alias Names: macrophage inflammatory protein 4-alpha; small inducible cytokine A26; CC chemokine IMAC; chemokine N1; thymic stroma chemokine-1; eotaxin-3; small inducible cytokine subfamily A (Cys-Cys), member 26; chemokine (C-C motif) ligand 26

CCL26, C-C motif chemokine ligand 26 Target Infomation by Species

  • human iconHuman
  • mouse iconMouse
  • rat iconRat

Human CCL26 Target Information

  • Target Symbol: CCL26, C-C motif chemokine ligand 26
  • Alias:
    • C-C motif chemokine 26
    • CC chemokine IMAC
    • chemokine (C-C motif) ligand 26
    • chemokine N1
    • eotaxin-3
    • IMAC
    • macrophage inflammatory protein 4-alpha
    • MGC126714
    • MIP-4-alpha
    • MIP-4a
    • MIP-4alpha
    • SCYA26
    • small inducible cytokine A26
    • small inducible cytokine subfamily A (Cys-Cys), member 26
    • small-inducible cytokine A26
    • thymic stroma chemokine-1
    • TSC-1
  • NCBI_Gene: 10344
  • UniProtKB: Q9Y258

Human CCL26 Predicted Functions

Enables CCR3 chemokine receptor binding activity; CX3C chemokine receptor binding activity; and chemokine activity. Involved in several processes, including leukocyte chemotaxis; positive regulation of GTPase activity; and positive regulation of actin filament polymerization. Located in extracellular space. Implicated in asthma; eosinophilic esophagitis; and rhinitis. Biomarker of several diseases, including Churg-Strauss syndrome; dermatitis (multiple); eosinophilic esophagitis; eosinophilic gastroenteritis; and rhinitis (multiple).

Mouse Ccl26 Target Information

Mouse Ccl26 Predicted Functions

Predicted to enable CCR3 chemokine receptor binding activity; CX3C chemokine receptor binding activity; and chemokine activity. Predicted to be involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and positive regulation of ERK1 and ERK2 cascade. Predicted to be active in extracellular space. Is expressed in several structures, including aorta; bone marrow; brain; genitourinary system; and respiratory system. Human ortholog(s) of this gene implicated in asthma; eosinophilic esophagitis; and rhinitis. Orthologous to human CCL26 (C-C motif chemokine ligand 26).

Rat Ccl26 Target Information

  • Target Symbol: Ccl26, C-C motif chemokine ligand 26
  • Alias:
    • C-C motif chemokine 26
    • chemokine (C-C motif) ligand 26
    • LOC685958
    • similar to eotaxin 3-like
  • NCBI_Gene: 685958
  • UniProtKB: D3ZQG2

Rat Ccl26 Predicted Functions

Predicted to enable CCR3 chemokine receptor binding activity; CX3C chemokine receptor binding activity; and chemokine activity. Involved in response to organonitrogen compound. Predicted to be active in extracellular space. Used to study asthma. Human ortholog(s) of this gene implicated in asthma; eosinophilic esophagitis; and rhinitis. Orthologous to human CCL26 (C-C motif chemokine ligand 26).

Related products