Name: Human Chitinase 3-like 1/CHI3L1 ELISA Kit
SKU: EK1164
Availability: InStock

EK1164

Human Chitinase 3-like 1/CHI3L1 ELISA Kit

$245.00 – $350.00

Related Target	CHI3L1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL (prediluted)
Sensitivity	1.10 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	80% – 110%
Average Recovery	92%

ELISA Kit Detail Information

Related Target	CHI3L1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL (prediluted)
Sensitivity	1.10 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	80% – 110%
Average Recovery	92%
Intra Precision	1.9% – 2.6%
Inter Precision	3.2% – 5.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CHI3L1 monoclonal antibody Human CHI3L1 freeze-dried standard CHI3L1 detect Antibody HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CHI3L1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CHI3L1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of CHI3L1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CHI3L1

CHI3L1 Target Infomation Overview

Target Symbol: CHI3L1, chitinase 3 like 1
Gene Groups: Chitinases
Alias: GP39; YKL40; YK-40
Alias Names: cartilage glycoprotein-39; chitinase 3-like 1 (cartilage glycoprotein-39)

CHI3L1, chitinase 3 like 1 Target Infomation by Species

Human
Mouse
Rat

Human CHI3L1 Target Information

Target Symbol: CHI3L1, chitinase 3 like 1
Alias:
- 39 kDa synovial protein
- ASRT7
- cartilage glycoprotein 39
- CGP-39
- chitinase 3-like 1 (cartilage glycoprotein-39)
- chitinase-3-like protein 1
- DKFZp686N19119
- FLJ38139
- GP-39
- GP39
- HC-gp39
- hCGP-39
- HCGP-3P
- YK-40
- YKL-40
- YKL40
- YYL-40
NCBI_Gene: 1116
UniProtKB: P36222

Human CHI3L1 Predicted Functions

Enables chitin binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; response to interleukin-1; and response to interleukin-6. Located in extracellular space. Implicated in asthma and schizophrenia. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bone cancer (multiple); lung disease (multiple); and reproductive organ cancer (multiple).

Mouse Chil1 Target Information

Target Symbol: Chil1, chitinase-like 1
Alias:
- AW208766
- Brp39
- Chi3l1
- chitinase 3-like 1
- expressed sequence AW208766
- glycoprotein 39
- Gp39
NCBI_Gene: 12654

Mouse Chil1 Predicted Functions

Predicted to enable chitin binding activity. Involved in positive regulation of interleukin-8 production; positive regulation of protein kinase B signaling; and positive regulation of protein phosphorylation. Located in endoplasmic reticulum. Is expressed in alimentary system; limb; and skeleton. Human ortholog(s) of this gene implicated in asthma and schizophrenia. Orthologous to human CHI3L1 (chitinase 3 like 1).

Rat Chi3l1 Target Information

Target Symbol: Chi3l1, chitinase 3 like 1
Alias:
- cartilage glycoprotein 39
- CGP-39
- Chil1
- chitinase 3-like 1
- chitinase 3-like 1 (cartilage glycoprotein-39)
- chitinase-3-like protein 1
- GP-39
- MGC109420
NCBI_Gene: 89824
- UniprotKB: A4LA56
- UniprotKB: Q9WTV1

Rat Chi3l1 Predicted Functions

Predicted to enable chitin binding activity. Involved in cellular response to interleukin-1; cellular response to tumor necrosis factor; and inflammatory response. Located in extracellular space. Biomarker of hypertension. Human ortholog(s) of this gene implicated in asthma and schizophrenia. Orthologous to human CHI3L1 (chitinase 3 like 1).

Citations for Human Chitinase 3-like 1/CHI3L1 ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.