Human G-CSF High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSCSF3
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity0.19 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery94% – 119%
Average Recovery1.06
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 50 μL;cell culture supernatant: 100 μL
Sensitivity0.19 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery94% – 119%
Average Recovery1.06
Intra Precision4.1% – 4.8%
Inter-Precision4.4% – 4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- G-CSF monoclonal antibody
Human G-CSF high sensitivity freeze-dried standard
G-CSF detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human G-CSF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the G-CSF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of G-CSF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CSF3

CSF3 Target Infomation Overview

  • Target Symbol: CSF3, colony stimulating factor 3
  • Gene Groups: Receptor ligands
  • Alias: MGC45931
  • Previous Names: GCSF; G-CSF; C17orf33
  • Alias Names: granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte)

CSF3, colony stimulating factor 3 Target Infomation by Species

HumanMouseRat

Human CSF3 Target Information

  • Target Symbol: CSF3, colony stimulating factor 3
  • Alias:
    • C17orf33
    • chromosome 17 open reading frame 33
    • colony stimulating factor 3 (granulocyte)
    • CSF3OS
    • filgrastim
    • G-CSF
    • GCSF
    • granulocyte colony-stimulating factor
    • granulocyte-colony stimulating factor
    • lenograstim
    • MGC45931
    • pluripoietin
  • NCBI_Gene: 1440
  • UniProtKB: P09919

Human CSF3 Predicted Functions

Enables cytokine activity and enzyme binding activity. Involved in several processes, including positive regulation of cytoskeleton organization; positive regulation of intracellular signal transduction; and positive regulation of protein phosphorylation. Predicted to be located in endocytic vesicle lumen; extracellular region; and lysosomal lumen. Predicted to be active in extracellular space. Implicated in several diseases, including allergic cutaneous vasculitis; artery disease (multiple); ischemia (multiple); leukemia (multiple); and lung disease (multiple). Biomarker of COVID-19; bacterial pneumonia; and myeloid leukemia.

Mouse Csf3 Target Information

Mouse Csf3 Predicted Functions

Enables granulocyte colony-stimulating factor receptor binding activity. Acts upstream of or within positive regulation of myeloid cell differentiation. Predicted to be located in endocytic vesicle lumen and extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including brain; dorsal aorta; liver; reproductive system; and yolk sac. Human ortholog(s) of this gene implicated in several diseases, including allergic cutaneous vasculitis; artery disease (multiple); ischemia (multiple); leukemia (multiple); and lung disease (multiple). Orthologous to human CSF3 (colony stimulating factor 3).

Rat Csf3 Target Information

  • Target Symbol: Csf3, colony stimulating factor 3
  • Alias:
    • colony stimulating factor 3 (granulocyte)
    • Gcsf
    • granulocyte colony-stimulating factor
  • NCBI_Gene: 25610
  • UniProtKB: P97712

Rat Csf3 Predicted Functions

Enables growth factor activity. Involved in several processes, including cellular response to lipopolysaccharide; negative regulation of neuron death; and response to ethanol. Located in extracellular space. Biomarker of cystitis; lung disease; and middle cerebral artery infarction. Human ortholog(s) of this gene implicated in several diseases, including allergic cutaneous vasculitis; artery disease (multiple); ischemia (multiple); leukemia (multiple); and lung disease (multiple). Orthologous to human CSF3 (colony stimulating factor 3).

CSF3 Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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