Human IL-1β High Sensitivity ELISA Kit

$380.00$480.00

TARGETSIL1B
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.02 pg/mL
Range0.19 pg/mL – 12 pg/mL
Assay Time3.5 h
Recovery93% – 112%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.02 pg/mL
Range0.19 pg/mL – 12 pg/mL
Assay Time3.5 h
Recovery93% – 112%
Average Recovery1.02
Intra Precision4.5% – 5.6%
Inter-Precision2.3% – 8.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1β monoclonal antibody
Human IL-1β freeze-dried standard
IL-1β detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-1β antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-1β present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL1B

IL1B Target Infomation Overview

  • Target Symbol: IL1B, interleukin 1 beta
  • Gene Groups: Interleukins
  • Alias: IL1F2; IL-1B; IL1-BETA

IL1B, interleukin 1 beta Target Infomation by Species

HumanMouseRat

Human IL1B Target Information

  • Target Symbol: IL1B, interleukin 1 beta
  • Alias:
    • catabolin
    • IL-1
    • IL-1 beta
    • IL1-BETA
    • IL1beta
    • IL1F2
    • interleukin 1, beta
    • interleukin 1beta
    • interleukin-1 beta
    • preinterleukin 1 beta
    • pro-interleukin-1-beta
  • NCBI_Gene: 3553
  • UniProtKB: P01584

Human IL1B Predicted Functions

Enables cytokine activity; integrin binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of nitrogen compound metabolic process; and regulation of signal transduction. Acts upstream of or within cellular response to organic substance and cellular response to xenobiotic stimulus. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bronchial disease (multiple); lung disease (multiple); and nose disease (multiple).

Mouse Il1b Target Information

Mouse Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

Rat Il1b Target Information

Rat Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

IL1B Target News

[catlist tags=”il1b” template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

2.6
Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sjögren’s syndrome
MICROBIOLOGY AND IMMUNOLOGY
5.9
Selenium-Enriched Soybean Peptides as Novel Organic Selenium Compound Supplements: Inhibition of Occupational Air Pollution Exposure-Induced Apoptosis in Lung Epithelial Cells
Nutrients
4.927
Confusoside from Anneslea fragrans Alleviates Acetaminophen-Induced Liver Injury in HepG2 via PI3K-CASP3 Signaling Pathway
MOLECULES
6.208
Glucokinase Inactivation Ameliorates Lipid Accumulation and Exerts Favorable Effects on Lipid Metabolism in Hepatocytes
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
5.561
Ultra-High Hydrostatic Pressure Pretreatment on White Que Zui Tea: Chemical Constituents, Antioxidant, Cytoprotective, and Anti-Inflammatory Activities
Foods
3.1
The Effect of an Extract of Sappanwood, Protosappanin A and Protosappanin B on Osteogenesis in Periodontitis
Frontiers in Bioscience-Landmark
2.7
TIPE2 regulates periodontal inflammation by inhibiting NF-κB p65 phosphorylation
Journal of Applied Oral Science
2.264
Tilianin Protects against Nonalcoholic Fatty Liver Disease in Early Obesity Mice
BIOLOGICAL & PHARMACEUTICAL BULLETIN
2.367
Home-based exercise alters pulmonary function and cellular stress markers in overweight middle-aged men during covid-19 Home quarantine
BMC Sports Science Medicine and Rehabilitation
4.546
Gegen Qinlian standard decoction alleviated irinotecan-induced diarrhea via PI3K/AKT/NF-κB axis by network pharmacology prediction and experimental validation combination
Chinese Medicine
6.376
4-Octyl itaconate attenuates glycemic deterioration by regulating macrophage polarization in mouse models of type 1 diabetes
MOLECULAR MEDICINE
3.247
Hyperoside exerts protective effects against anticardiolipin antibody-induced recurrent pregnancy loss in vivo and in vitro
HUMAN & EXPERIMENTAL TOXICOLOGY
3.654
Kaempferol Reverses Acute Kidney Injury in Septic Model by Inhibiting NF-κB/AKT Signaling Pathway
JOURNAL OF FOOD BIOCHEMISTRY
2.5
Protective effect of small extracellular vesicles (EVs) derived from ACE2-modified human umbilical cord mesenchymal stem cells against renal ischemia–reperfusion injury
NEPHROLOGY
8.755
The sodium new houttuyfonate suppresses NSCLC via activating pyroptosis through TCONS-14036/miR-1228-5p/PRKCDBP pathway
CELL PROLIFERATION
3.1
CircTDRD9 contributes to sepsis-induced acute lung injury by enhancing the expression of RAB10 via directly binding to miR-223-3p
SHOCK
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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