Human/Mouse/Rat TGF-β2 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	TGFB2
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.33 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	88% – 120%
Average Recovery	1.07

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.33 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	88% – 120%
Average Recovery	1.07
Intra Precision	2.7% – 4.5%
Inter-Precision	3.3% – 4.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TGF-β2 monoclonal antibody Human /Mouse /Rat TGF-β2 freeze-dried standard TGF-β2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human /mouse /rat TGF-β2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TGF-β2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TGF-β2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

TGFB2

TGFB2 Target Infomation Overview

Target Symbol: TGFB2, transforming growth factor beta 2
Gene Groups: Transforming growth factor beta family
Alias Names: prepro-transforming growth factor beta-2; transforming growth factor, beta 2

TGFB2, transforming growth factor beta 2 Target Infomation by Species

HumanMouseRat

Human TGFB2 Target Information

Target Symbol: TGFB2, transforming growth factor beta 2
Alias:
- BSC-1 cell growth inhibitor
- cetermin
- G-TSF
- glioblastoma-derived T-cell suppressor factor
- LDS4
- MGC116892
- polyergin
- prepro-transforming growth factor beta-2
- TGF-beta-2
- TGF-beta2
- transforming growth factor beta-2
- transforming growth factor beta-2 proprotein
- transforming growth factor, beta 2
NCBI_Gene: 7042
UniProtKB: P61812

Human TGFB2 Predicted Functions

Enables amyloid-beta binding activity; protein homodimerization activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including animal organ development; cell surface receptor signaling pathway; and positive regulation of signal transduction. Located in collagen-containing extracellular matrix and extracellular space. Implicated in Loeys-Dietz syndrome 4 and colorectal cancer. Biomarker of colon carcinoma; colorectal cancer; and liver cirrhosis.

Mouse Tgfb2 Target Information

Target Symbol: Tgfb2, transforming growth factor, beta 2
Alias:
- BB105277
- expressed sequence BB105277
- Tgf-beta2
- Tgfb-2
NCBI_Gene: 21808

Mouse Tgfb2 Predicted Functions

Predicted to enable several functions, including amyloid-beta binding activity; protein homodimerization activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including animal organ development; regulation of epithelial to mesenchymal transition involved in endocardial cushion formation; and tube morphogenesis. Acts upstream of or within several processes, including animal organ development; neuron differentiation; and positive regulation of apoptotic process. Located in several cellular components, including axon; endosome; and neuronal cell body. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart; and sensory organ. Used to study Loeys-Dietz syndrome. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 4 and colorectal cancer. Orthologous to human TGFB2 (transforming growth factor beta 2).

Rat Tgfb2 Target Information

Target Symbol: Tgfb2, transforming growth factor, beta 2
Alias:
- TGF beta 2 protein
- TGF-B2
- TGF-beta-2
- transforming growth factor beta-2
- transforming growth factor beta-2 proprotein
NCBI_Gene: 81809

Rat Tgfb2 Predicted Functions

Enables identical protein binding activity and protein N-terminus binding activity. Involved in several processes, including animal organ development; negative regulation of release of sequestered calcium ion into cytosol; and regulation of apoptotic process. Located in several cellular components, including basement membrane; secretory granule; and trans-Golgi network. Biomarker of diabetic neuropathy; myocardial infarction; and osteochondrodysplasia. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 4 and colorectal cancer. Orthologous to human TGFB2 (transforming growth factor beta 2).