Mouse HGF ELISA Kit

$350.00$450.00

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TARGETSSOS1
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity22.20 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery91% – 118%
Average Recovery1.03
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity22.20 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery91% – 118%
Average Recovery1.03
Intra Precision3.4% – 4.2%
Inter-Precision3.2% – 4.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Mouse HGF monoclonal antibody
Mouse HGF freeze-dried standard
Mouse HGF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse HGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, HGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of HGF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

SOS1

SOS1 Target Infomation Overview

  • Target Symbol: SOS1, SOS Ras/Rac guanine nucleotide exchange factor 1
  • Gene Groups: Pleckstrin homology domain containing; Dbl family Rho GEFs
  • Alias: HGF; GF1
  • Previous Names: GINGF
  • Alias Names: gingival fibromatosis, hereditary, 1; son of sevenless homolog 1 (Drosophila)

SOS1, SOS Ras/Rac guanine nucleotide exchange factor 1 Target Infomation by Species

HumanMouseRat

Human SOS1 Target Information

  • Target Symbol: SOS1, SOS Ras/Rac guanine nucleotide exchange factor 1
  • Alias:
    • GF1
    • GGF1
    • GINGF
    • gingival fibromatosis, hereditary, 1
    • guanine nucleotide exchange factor
    • HGF
    • NS4
    • son of sevenless homolog 1
    • SOS-1
  • NCBI_Gene: 6654
  • UniProtKB: Q07889

Human SOS1 Predicted Functions

Enables guanyl-nucleotide exchange factor activity. Predicted to be involved in axon guidance; cell surface receptor signaling pathway; and leukocyte migration. Predicted to act upstream of or within several processes, including animal organ development; regulation of T cell activation; and transmembrane receptor protein tyrosine kinase signaling pathway. Located in cytoplasm. Implicated in Noonan syndrome; Noonan syndrome 4; and gingival fibromatosis. Biomarker of cervical cancer and prostate cancer.

Mouse Sos1 Target Information

Mouse Sos1 Predicted Functions

Enables identical protein binding activity. Predicted to be involved in several processes, including induction of bacterial agglutination; negative regulation of interleukin-2 production; and regulation of phagocytosis. Predicted to act upstream of or within innate immune response and respiratory gaseous exchange by respiratory system. Located in cytoplasm. Is expressed in lung; otic capsule; and skeleton. Human ortholog(s) of this gene implicated in asthma; lung disease (multiple); respiratory syncytial virus infectious disease; and rhinitis. Orthologous to human SFTPD (surfactant protein D).

Rat Sos1 Target Information

Rat Sos1 Predicted Functions

Enables SH3 domain binding activity. Involved in Ras protein signal transduction; neurotrophin TRK receptor signaling pathway; and positive regulation of signal transduction. Located in neuronal cell body; plasma membrane; and postsynaptic density. Human ortholog(s) of this gene implicated in Noonan syndrome; Noonan syndrome 4; and gingival fibromatosis. Orthologous to human SOS1 (SOS Ras/Rac guanine nucleotide exchange factor 1).

SOS1 Target News

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