Mouse HGF ELISA Kit
$350.00 – $450.00
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
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Sample Volume | 20 μL |
Sensitivity | 22.20 pg/mL |
Range | 0.16 ng/mL – 10 ng/mL |
Assay Time | 3.5 h |
Recovery | 91% – 118% |
Average Recovery | 1.03 |
Intra Precision | 3.4% – 4.2% |
Inter-Precision | 3.2% – 4.3% |
Platform | ELISA |
Plate | Detachable 96-well plate |
Size | 96T/48T |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Mouse HGF monoclonal antibody Mouse HGF freeze-dried standard Mouse HGF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse HGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, HGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of HGF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
SOS1
SOS1 Target Infomation Overview
- Target Symbol: SOS1, SOS Ras/Rac guanine nucleotide exchange factor 1
- Gene Groups: Pleckstrin homology domain containing; Dbl family Rho GEFs
- Alias: HGF; GF1
- Previous Names: GINGF
- Alias Names: gingival fibromatosis, hereditary, 1; son of sevenless homolog 1 (Drosophila)
SOS1, SOS Ras/Rac guanine nucleotide exchange factor 1 Target Infomation by Species
Human SOS1 Target Information
- Target Symbol: SOS1, SOS Ras/Rac guanine nucleotide exchange factor 1
- Alias:
- GF1
- GGF1
- GINGF
- gingival fibromatosis, hereditary, 1
- guanine nucleotide exchange factor
- HGF
- NS4
- son of sevenless homolog 1
- SOS-1
- NCBI_Gene: 6654
- UniProtKB: Q07889
Human SOS1 Predicted Functions
Enables guanyl-nucleotide exchange factor activity. Predicted to be involved in axon guidance; cell surface receptor signaling pathway; and leukocyte migration. Predicted to act upstream of or within several processes, including animal organ development; regulation of T cell activation; and transmembrane receptor protein tyrosine kinase signaling pathway. Located in cytoplasm. Implicated in Noonan syndrome; Noonan syndrome 4; and gingival fibromatosis. Biomarker of cervical cancer and prostate cancer.
Mouse Sos1 Target Information
- Target Symbol: Sos1, SOS Ras/Rac guanine nucleotide exchange factor 1
- Alias:
- AI573415
- expressed sequence AI573415
- Sfpd
- Sftp4
- SP-D
- surfactant-associated protein 4
- NCBI_Gene: 20662
Mouse Sos1 Predicted Functions
Enables identical protein binding activity. Predicted to be involved in several processes, including induction of bacterial agglutination; negative regulation of interleukin-2 production; and regulation of phagocytosis. Predicted to act upstream of or within innate immune response and respiratory gaseous exchange by respiratory system. Located in cytoplasm. Is expressed in lung; otic capsule; and skeleton. Human ortholog(s) of this gene implicated in asthma; lung disease (multiple); respiratory syncytial virus infectious disease; and rhinitis. Orthologous to human SFTPD (surfactant protein D).
Rat Sos1 Target Information
- Target Symbol: Sos1, SOS Ras/Rac guanine nucleotide exchange factor 1
- Alias:
- LOC313845
- Son of sevenless homolog 1
- Son of sevenless homolog 1 (Drosophila)
- NCBI_Gene: 313845
Rat Sos1 Predicted Functions
Enables SH3 domain binding activity. Involved in Ras protein signal transduction; neurotrophin TRK receptor signaling pathway; and positive regulation of signal transduction. Located in neuronal cell body; plasma membrane; and postsynaptic density. Human ortholog(s) of this gene implicated in Noonan syndrome; Noonan syndrome 4; and gingival fibromatosis. Orthologous to human SOS1 (SOS Ras/Rac guanine nucleotide exchange factor 1).
SOS1 Target News
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