Mouse IL-1β ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL1B
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant
Sample Volume	Serum, plasma: 10 μL；cell culture supernatant: 100 μL
Sensitivity	4.82 pg/mL
Range	23.44 pg/mL – 1500 pg/mL
Assay Time	3.5 h
Recovery	83% – 96%
Average Recovery	91%

Sample Type	Serum, plasma, cell culture supernatant
Sample Volume	Serum, plasma: 10 μL；cell culture supernatant: 100 μL
Sensitivity	4.82 pg/mL
Range	23.44 pg/mL – 1500 pg/mL
Assay Time	3.5 h
Recovery	83% – 96%
Average Recovery	91%
Intra Precision	6.2% – 7.2%
Inter-Precision	3.9% – 8.0%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	IL-1β Microplate IL-1β Standard IL-1β Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Plate Covers
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IL-1β has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IL-1β are pipetted into the wells and IL-1β present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.

Targets

IL1B

IL1B Target Infomation Overview

Target Symbol: IL1B, interleukin 1 beta
Gene Groups: Interleukins
Alias: IL1F2; IL-1B; IL1-BETA

IL1B, interleukin 1 beta Target Infomation by Species

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Human IL1B Target Information

Target Symbol: IL1B, interleukin 1 beta
Alias:
- catabolin
- IL-1
- IL-1 beta
- IL1-BETA
- IL1beta
- IL1F2
- interleukin 1, beta
- interleukin 1beta
- interleukin-1 beta
- preinterleukin 1 beta
- pro-interleukin-1-beta
NCBI_Gene: 3553
UniProtKB: P01584

Human IL1B Predicted Functions

Enables cytokine activity; integrin binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of nitrogen compound metabolic process; and regulation of signal transduction. Acts upstream of or within cellular response to organic substance and cellular response to xenobiotic stimulus. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bronchial disease (multiple); lung disease (multiple); and nose disease (multiple).

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Mouse Il1b Target Information

Target Symbol: Il1b, interleukin 1 beta
Alias:
- IL-1B
- Il-1b
- IL-1beta
NCBI_Gene: 16176
- UniprotKB: P10749
- UniprotKB: Q3TI17

Mouse Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

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Rat Il1b Target Information

Target Symbol: Il1b, interleukin 1 beta
Alias:
- IL-1 beta
- IL-1F2
- interleukin-1 beta
NCBI_Gene: 24494
- UniprotKB: Q5BKB0
- UniprotKB: Q63264

Rat Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

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