Mouse IL-1β ELISA Kit

$350.00$450.00

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TARGETSIL1B
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL;cell culture supernatant: 100 μL
Sensitivity1.45 pg/mL
Range23.44 pg/mL – 1500 pg/mL
Assay Time3.5 h
Recovery83% – 109%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL;cell culture supernatant: 100 μL
Sensitivity1.45 pg/mL
Range23.44 pg/mL – 1500 pg/mL
Assay Time3.5 h
Recovery83% – 109%
Average Recovery0.97
Intra Precision2.8% – 5.6%
Inter-Precision4.2% – 8.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- IL-1β monoclonal antibody
Mouse IL-1β freeze-dried standard
IL-1β detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-1β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-1β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL1B

IL1B Target Infomation Overview

  • Target Symbol: IL1B, interleukin 1 beta
  • Gene Groups: Interleukins
  • Alias: IL1F2; IL-1B; IL1-BETA

IL1B, interleukin 1 beta Target Infomation by Species

HumanMouseRat

Human IL1B Target Information

  • Target Symbol: IL1B, interleukin 1 beta
  • Alias:
    • catabolin
    • IL-1
    • IL-1 beta
    • IL1-BETA
    • IL1beta
    • IL1F2
    • interleukin 1, beta
    • interleukin 1beta
    • interleukin-1 beta
    • preinterleukin 1 beta
    • pro-interleukin-1-beta
  • NCBI_Gene: 3553
  • UniProtKB: P01584

Human IL1B Predicted Functions

Enables cytokine activity; integrin binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of nitrogen compound metabolic process; and regulation of signal transduction. Acts upstream of or within cellular response to organic substance and cellular response to xenobiotic stimulus. Located in extracellular space. Implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bronchial disease (multiple); lung disease (multiple); and nose disease (multiple).

Mouse Il1b Target Information

Mouse Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

Rat Il1b Target Information

Rat Il1b Predicted Functions

Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

IL1B Target News

    2.6
    Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sjögren’s syndrome
    MICROBIOLOGY AND IMMUNOLOGY
    3.7
    Involvement of estrogen receptor activation in kaempferol-3-O-glucoside’s protection against aging-related cognition impairment and microglial inflammation
    EXPERIMENTAL CELL RESEARCH
    3.3
    GPR34 knockdown relieves cognitive deficits and suppresses neuroinflammation in Alzheimer’s disease via the ERK/NF-κB signal
    NEUROSCIENCE
    9.417
    Engineered inhalable nanocatalytic therapeutics for Parkinson’s disease by inducing mitochondrial autophagy
    MATERIALS & DESIGN
    4
    Network pharmacology and in vivo studies reveal the neuroprotective effects of paeoniflorin on Alzheimer’s disease
    Heliyon
    6.2
    Identification of Clec7a as the therapeutic target of rTMS in alleviating Parkinson’s disease: targeting neuroinflammation
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
    7.7
    GLI1 facilitates collagen-induced arthritis in mice by collaborative regulation of DNA methyltransferases
    eLife
    6.058
    Heat shock factor 1 promotes neurite outgrowth and suppresses inflammation in the severed spinal cord of geckos
    Neural Regeneration Research
    6.1
    Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis
    Neural Regeneration Research
    3.4
    TIFA contributes to periodontitis in diabetic mice via activating the NF‑κB signaling pathway
    Molecular Medicine Reports
    5.4
    Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
    4.967
    Effects of Pine Pollen Polysaccharides and Sulfated Polysaccharides on Ulcerative Colitis and Gut Flora in Mice
    Polymers
    4.6
    Triterpenoid and Coumarin Isolated from Astilbe grandis with Anti-Inflammatory Effects through Inhibiting the NF-κB Pathway in LPS-Induced RAW264.7 Cells
    MOLECULES
    5.4
    UV/H2O2-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity
    Marine Drugs
    4.927
    Synergistic Antitumor Effect of Grifola frondose Polysaccharide—Protein Complex in Combination with Cyclophosphamide in H22 Tumor-Bearing Mice
    MOLECULES
    4.5
    Profile of Pancreatic and Ileal Microbiota in Experimental Acute Pancreatitis
    Microorganisms
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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