Mouse TNF-α High Sensitivity ELISA Kit

$395.00$485.00

TARGETS TNF
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant
Sample Volume Serum/plasma: 10 μL, cell culture supernatant:100 μL
Sensitivity 0.67 pg/mL
Range 1.56 pg/mL – 100 pg/mL
Assay Time 3.5 h
Recovery 78% – 113%
Average Recovery 95%
Sample Type Serum, plasma, cell culture supernatant
Sample Volume Serum/plasma: 10 μL, cell culture supernatant:100 μL
Sensitivity 0.67 pg/mL
Range 1.56 pg/mL – 100 pg/mL
Assay Time 3.5 h
Recovery 78% – 113%
Average Recovery 95%
Intra Precision 5.0% – 6.1%
Inter-Precision 2.9% – 4.5%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody
Mouse TNF-α freeze-dried standard
TNF-α detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse TNF-α antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the TNF-α present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TNF-α in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TNF

TNF Target Infomation Overview

  • Target Symbol: TNF, tumor necrosis factor
  • Gene Groups: Tumor necrosis factor superfamily
  • Alias: TNFSF2; DIF; TNF-alpha
  • Previous Names: TNFA
  • Alias Names: TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF, tumor necrosis factor Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human TNF Target Information

  • Target Symbol: TNF, tumor necrosis factor
  • Alias:
    • APC1 protein
    • cachectin
    • DIF
    • TNF superfamily, member 2
    • TNF-a
    • TNF-alpha
    • TNF, macrophage-derived
    • TNF, monocyte-derived
    • TNFA
    • TNFSF2
    • TNLG1F
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand 1F
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor-alpha
  • NCBI_Gene: 7124
  • UniProtKB: P01375

Human TNF Predicted Functions

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Tnf Target Information

Mouse Tnf Predicted Functions

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Tnf Target Information

  • Target Symbol: Tnf, tumor necrosis factor
  • Alias:
    • cachectin
    • LOC103694380
    • MGC124630
    • RATTNF
    • TNF-a
    • TNF-alpha
    • Tnfa
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor superfamily member 2
    • tumor necrosis factor superfamily, member 2
    • tumor necrosis factor-alpha
    • tumor necrosis factor-like
  • NCBI_Gene: 24835
  • UniProtKB: P16599

Rat Tnf Predicted Functions

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

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TNF Target News

[catlist tags=”tnf” template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

7.7
Bionic sulfated glycosaminoglycan-based hydrogel inspired by snail mucus promotes diabetic chronic wound healing via regulating macrophage polarization
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
9.3
Aberrant copper metabolism and hepatic inflammation cause neurological manifestations in a mouse model of Wilson’s disease
Journal of Neuroinflammation
4.8
Quercetin-primed BMSC-derived extracellular vesicles ameliorate chronic liver damage through miR-136-5p and GNAS/STAT3 signaling pathways
INTERNATIONAL IMMUNOPHARMACOLOGY
15.5
Enhancing protective immunity against bacterial infection via coating nano-Rehmannia glutinosa polysaccharide with outer membrane vesicles
Journal of Extracellular Vesicles
14.7
Glypican-3-targeted macrophages delivering drug-loaded exosomes offer efficient cytotherapy in mouse models of solid tumours
Nature Communications
6.6
Metabolic Reprogramming of CD4+ T Cells by Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuates Autoimmune Hepatitis Through Mitochondrial Protein Transfer
International Journal of Nanomedicine
13.9
Phased Ions-Release Bilayer-Guided Bone Regeneration Membrane with Nanostructure-Mediated Antibacterial Adhesion
Small Structures
11.7
Hybrid nanoparticle–mediated simultaneous ROS scavenging and STING activation improve the antitumor immunity of in situ vaccines
Science Advances
4.9
Targeted Delivery of STING Agonist via Albumin Nanoreactor Boosts Immunotherapeutic Efficacy against Aggressive Cancers
Pharmaceutics
8.3
Self-Healing Photothermal Nanotherapeutics for Enhanced Tumor Therapy through Triple Ferroptosis Amplification and Cascade Inflammation Inhibition
ACS Applied Materials & Interfaces
8.2
Serum amyloid A contributes to radiation-induced lung injury by activating macrophages through FPR2/Rac1/NF-κB pathway
International Journal of Biological Sciences
6.7
Ginsenoside Rg2 alleviates astrocyte inflammation and ameliorates the permeability of the Alzheimer’s disease related blood-brain barrier
PHYTOMEDICINE
10.6
A nanobody-guided multifunctional T cell engager promotes strong anti-tumor responses via synergistic immuno-photothermal effects
JOURNAL OF NANOBIOTECHNOLOGY
6
Capsaicin mitigates ventilator-induced lung injury by suppressing ferroptosis and maintaining mitochondrial redox homeostasis through SIRT3-dependent mechanisms
MOLECULAR MEDICINE
14.7
Localized light-triggered release macrophage cytopharmaceuticals containing O-nitrobenzyl group for enhanced solid tumor cell-chemotherapy
Acta Pharmaceutica Sinica B
3.4
QSOX1 exerts anti-inflammatory effects in sepsis-induced acute lung injury: regulation involving EGFR phosphorylation mediated M1 polarization of macrophages
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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