Powered by BiozEasyGo!™ Mouse CD6 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 10uL |
| Sensitivity | 2.21 pg/ml |
| Array Range | 10.94-700pg/ml |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
CD6
CD6 Target Infomation Overview
- Target Symbol: CD6, CD6 molecule
- Gene Groups: Scavenger receptor cysteine rich domain containing; CD molecules
- Alias: Tp120, CD6 antigen
CD6, CD6 molecule Target Infomation by Species
- Human
- Mouse
- Rat
Human CD6 Target Information
- Target Symbol: CD6, CD6 molecule
- Alias:
- CD6 antigen
- FLJ44171
- T-cell differentiation antigen CD6
- T12
- TP120
- NCBI_Gene: 923
- UniProtKB: P30203
人 Human CD6 Predicted Functions
Enables identical protein binding activity; lipopolysaccharide binding activity; and lipoteichoic acid binding activity. Involved in several processes, including heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; lipopolysaccharide-mediated signaling pathway; and positive regulation of cytokine production involved in inflammatory response. Located in T cell receptor complex and immunological synapse.
Mouse CD6 Target Information
- Target Symbol: Cd6, CD6 antigen
- Alias:
- OX52
- NCBI_Gene: 12511
Mouse CD6 Predicted Functions
Predicted to enable several functions, including identical protein binding activity; lipopolysaccharide binding activity; and lipoteichoic acid binding activity. Predicted to be involved in several processes, including heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; lipopolysaccharide-mediated signaling pathway; and positive regulation of macromolecule metabolic process. Predicted to be located in cell surface and immunological synapse. Predicted to be part of T cell receptor complex. Orthologous to human CD6 (CD6 molecule).
Rat CD6 Target Information
- Target Symbol: Cd6, CD6 antigen
- Alias:
- CD6 antigen
- CD6 antigen Tp120
- CD6 antigen, Tp120
- LOC100911366
- MGC108551
- OX52
- T-cell differentiation antigen CD6
- T-cell differentiation antigen CD6-like
- NCBI_Gene: 25752
Rat CD6 Predicted Functions
Enables protein kinase binding activity. Involved in positive regulation of protein phosphorylation. Located in cell surface. Orthologous to human CD6 (CD6 molecule).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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