Powered by BiozEasyGo!™ Mouse IFNGR1/CD119 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 10uL |
| Sensitivity | 8.65 pg/ml |
| Array Range | 15.63-1000pg/ml |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
IFNGR1
IFNGR1 Target Infomation Overview
- Target Symbol: IFNGR1, interferon gamma receptor 1
- Gene Groups: Interferon receptors; CD molecules
- Alias: CD119
- Alias Names: IFNGR
IFNGR1, interferon gamma receptor 1 Target Infomation by Species
- Human
- Mouse
- Rat
人 Human IFNGR1 Target Information
- Target Symbol: IFNGR1, interferon gamma receptor 1
- Alias:
- antiviral protein, type 2
- AVP, type 2
- CD119
- CD119 antigen
- CDw119
- FLJ45734
- IFN-gamma receptor 1
- IFN-gamma-R-alpha
- IFN-gamma-R1
- IFNGR
- IMD27A
- IMD27B
- immune interferon receptor 1
- interferon-gamma receptor alpha chain
- NCBI_Gene: 3459
- UniProtKB: P15260
Human IFNGR1 Predicted Functions
Enables type II interferon receptor activity. Involved in type II interferon-mediated signaling pathway and type III interferon-mediated signaling pathway. Is active in plasma membrane. Implicated in asthma; hepatitis B; immunodeficiency 27A; immunodeficiency 27B; and tuberculosis. Biomarker of arteriosclerosis; periodontitis; pulmonary hypertension; and uveitis.
Mouse Ifngr1 Target Information
- Target Symbol: Ifngr1, interferon gamma receptor 1
- Alias:
- CD119
- Ifgr
- IFN-gamma R
- IFN-gammaR
- Ifngr
- Nktar
- NCBI_Gene: 15979
Mouse Ifngr1 Predicted Functions
Predicted to enable type II interferon receptor activity. Involved in glial cell activation; negative regulation of amyloid-beta clearance; and positive regulation of macromolecule metabolic process. Acts upstream of or within defense response to virus. Predicted to be located in dendrite and vesicle. Predicted to be active in plasma membrane. Is expressed in several structures, including alimentary system; bone; cerebral cortex; female reproductive system; and liver. Used to study osteoporosis. Human ortholog(s) of this gene implicated in asthma; hepatitis B; immunodeficiency 27A; immunodeficiency 27B; and tuberculosis. Orthologous to human IFNGR1 (interferon gamma receptor 1).
Rat Ifngr1 Target Information
- Target Symbol: Ifngr1, interferon gamma receptor 1
- Alias:
- Ifngr
- interferon gamma receptor
- NCBI_Gene: 116465
Rat Ifngr1 Predicted Functions
Predicted to enable type II interferon receptor activity. Predicted to be involved in several processes, including glial cell activation; interferon-mediated signaling pathway; and positive regulation of macromolecule metabolic process. Predicted to act upstream of or within defense response to virus. Located in dendrite and vesicle. Human ortholog(s) of this gene implicated in asthma; hepatitis B; immunodeficiency 27A; immunodeficiency 27B; and tuberculosis. Orthologous to human IFNGR1 (interferon gamma receptor 1).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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