Powered by BiozEasyGo!™ Mouse IL-1R1/CD121a One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 10uL |
| Sensitivity | 5.47 pg/ml |
| Array Range | 62.50-4000pg/ml |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
IL1R1
IL1R1 Target Infomation Overview
- Target Symbol: IL1R1, interleukin 1 receptor type 1
- Gene Groups: I-set domain containing; Interleukin receptors; TIR domain containing; CD molecules
- Alias: D2S1473; CD121A; IL1R; IL1RA
- Alias Names: interleukin 1 receptor, type I; interleukin-1 receptor, type I
IL1R1, interleukin 1 receptor type 1 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL1R1 Target Information
- Target Symbol: IL1R1, interleukin 1 receptor type 1
- Alias:
- antigen CD121a
- CD121 antigen-like family member A
- CD121A
- CRMO3
- D2S1473
- IL-1R-1
- IL-1R-alpha
- IL-1RT-1
- IL-1RT1
- IL1R
- IL1RA
- interleukin 1 receptor alpha, type I
- interleukin 1 receptor, type I
- interleukin receptor 1
- interleukin-1 receptor alpha
- interleukin-1 receptor type 1
- interleukin-1 receptor type I
- P80
- NCBI_Gene: 3554
- UniProtKB: P14778
Human IL1R1 Predicted Functions
Enables interleukin-1 receptor activity and platelet-derived growth factor receptor binding activity. Involved in interleukin-1-mediated signaling pathway; positive regulation of cytokine production; and positive regulation of signal transduction. Located in membrane. Is active in plasma membrane. Implicated in chronic recurrent multifocal osteomyelitis; systemic scleroderma; and type 1 diabetes mellitus. Biomarker of COVID-19; cholesteatoma of middle ear; and severe acute respiratory syndrome.
Mouse Il1r1 Target Information
- Target Symbol: Il1r1, interleukin 1 receptor, type I
- Alias:
- CD121a
- IL-1 receptor alpha chain
- IL-1R-1
- IL-1R-alpha
- IL-1R1
- IL-1RT-1
- IL-1RT1
- IL-iR
- Il1r-1
- NCBI_Gene: 16177
Mouse Il1r1 Predicted Functions
Enables interleukin-1 binding activity; interleukin-1 receptor activity; and protease binding activity. Involved in interleukin-1-mediated signaling pathway. Acts upstream of or within several processes, including cytokine-mediated signaling pathway; positive regulation of interleukin-1-mediated signaling pathway; and positive regulation of neutrophil extravasation. Located in external side of plasma membrane. Is expressed in aorta-gonad-mesonephros; brain; liver; spleen; and uterus. Used to study Sjogren's syndrome and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in chronic recurrent multifocal osteomyelitis; systemic scleroderma; and type 1 diabetes mellitus. Orthologous to human IL1R1 (interleukin 1 receptor type 1).
Rat Il1r1 Target Information
- Target Symbol: Il1r1, interleukin 1 receptor, type I
- Alias:
- CD121 antigen-like family member A
- IL-1R-1
- IL-1R-alpha
- IL-1RT-1
- IL-1RT1
- interleukin 1 beta receptor type 1 short transmembrane form
- interleukin 1 beta receptor type 1 soluble form
- interleukin 1 bete receptor type 1 long transmembrane form
- interleukin 1 receptor, type I
- interleukin-1 receptor alpha
- interleukin-1 receptor type 1
- interleukin-1 receptor type I
- p80
- NCBI_Gene: 25663
- UniProtKB: Q02955
Rat Il1r1 Predicted Functions
Enables ionotropic glutamate receptor binding activity. Involved in several processes, including interleukin-1-mediated signaling pathway; positive regulation of neuron migration; and response to odorant. Located in axon; cell surface; and extracellular space. Part of protein-containing complex. Biomarker of pulpitis. Human ortholog(s) of this gene implicated in chronic recurrent multifocal osteomyelitis; systemic scleroderma; and type 1 diabetes mellitus. Orthologous to human IL1R1 (interleukin 1 receptor type 1).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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