Powered by BiozEasyGo!™ Mouse IL2RG/CD132 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20uL |
| Sensitivity | 9.24 pg/mL |
| Array Range | 46.88-3000pg/mL |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
IL2RG
IL2RG Target Infomation Overview
- Target Symbol: IL2RG, interleukin 2 receptor subunit gamma
- Gene Groups: Interleukin receptors; Fibronectin type III domain containing; CD molecules
- Alias: CD132; SCIDX1; IMD4; CIDX
- Alias Names: severe combined immunodeficiency; combined immunodeficiency, X-linked; interleukin 2 receptor, gamma
IL2RG, interleukin 2 receptor subunit gamma Target Infomation by Species
- Human
- Mouse
- Rat
Human IL2RG Target Information
- Target Symbol: IL2RG, interleukin 2 receptor subunit gamma
- Alias:
- CD132
- CD132 antigen
- CIDX
- combined immunodeficiency, X-linked
- common cytokine receptor gamma chain
- common gamma-chain
- cytokine receptor common subunit gamma
- gamma(c)
- gammaC
- IL-2 receptor subunit gamma
- IL-2R subunit gamma
- IL-2RG
- IMD4
- interleukin 2 receptor, gamma
- interleukin 2 receptor, gamma (severe combined immunodeficiency)
- interleukin 2 receptor, gamma chain
- interleukin-2 receptor subunit gamma
- nonfunctional common cytokine receptor gamma chain
- P64
- SCIDX
- SCIDX1
- severe combined immunodeficiency
- NCBI_Gene: 3561
- UniProtKB: P31785
Human IL2RG Predicted Functions
Enables coreceptor activity and interleukin-15 receptor activity. Involved in cellular homeostasis; cytokine-mediated signaling pathway; and positive regulation of phagocytosis. Located in cell surface and nucleoplasm. Is active in plasma membrane. Implicated in X-linked severe combined immunodeficiency; combined T cell and B cell immunodeficiency; and severe combined immunodeficiency. Biomarker of COVID-19.
Mouse Il2rg Target Information
- Target Symbol: Il2rg, interleukin 2 receptor, gamma chain
- Alias:
- [g]c
- Ab2-183
- CD132
- Cd132
- common cytokine receptor gamma chain
- common gamma chain
- gamma C receptor
- gamma(c)
- gammaC
- gc
- <NCBI_Gene: 16186
Mouse Il2rg Predicted Functions
Enables interleukin-2 binding activity. Acts upstream of or within positive regulation of gene expression and positive regulation of lymphocyte differentiation. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; immune system; and liver and biliary system. Human ortholog(s) of this gene implicated in X-linked severe combined immunodeficiency; combined T cell and B cell immunodeficiency; and severe combined immunodeficiency. Orthologous to human IL2RG (interleukin 2 receptor subunit gamma).
Rat Il2rg Target Information
- Target Symbol: Il2rg, interleukin 2 receptor subunit gamma
- Alias:
- Ab2-183
- Cd132
- cytokine receptor common subunit gamma
- interleukin 2 receptor, gamma (severe combined immunodeficiency)
- interleukin 2 receptor, gamma chain
- NCBI_Gene: 140924
Rat Il2rg Predicted Functions
Predicted to enable coreceptor activity; interleukin-15 receptor activity; and interleukin-2 binding activity. Predicted to be involved in cellular homeostasis; cytokine-mediated signaling pathway; and positive regulation of phagocytosis. Predicted to act upstream of or within positive regulation of gene expression and positive regulation of lymphocyte differentiation. Predicted to be located in external side of plasma membrane and nucleoplasm. Predicted to be active in plasma membrane. Used to study X-linked severe combined immunodeficiency and combined T cell and B cell immunodeficiency. Human ortholog(s) of this gene implicated in X-linked severe combined immunodeficiency; combined T cell and B cell immunodeficiency; and severe combined immunodeficiency. Orthologous to human IL2RG (interleukin 2 receptor subunit gamma).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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