EasyGo!™ Mouse S100A1 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
Related Target | |
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Species | mouse |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 100uL(diluent) |
Sensitivity | 4.85 pg/ml |
Array Range | 31.25-2000pg/ml |
Assay Time | 90min |
Recovery | 70%-130% |
Average Recovery | / |
Intra Precision | < 10% |
Inter Precision | < 10% |
Plate | Detachable 96-well Plate |
Storage | 2-8℃ |
Delivery | blue ice |
Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
S100A1
S100A1 Target Infomation Overview
- Target Symbol: S100A1, S100 calcium binding protein A1
- Gene Groups: S100 calcium binding proteins; EF-hand domain containing
- Alias: S100-alpha, S100A
- Alias Names: S100 calcium-binding protein A1
S100A1, epidermal growth factor receptor Target Infomation by Species
- Human
- Mouse
- Rat
Human S100A1 Target Information
- Target Symbol: S100A1, S100 calcium binding protein A1
- Alias:
- protein S100-A1
- S-100 protein alpha chain
- S-100 protein subunit alpha
- S100
- S100 alpha
- S100 calcium-binding protein A1
- S100 protein, alpha polypeptide
- S100-alpha
- S100A
- NCBI_Gene: 6271
- UniProtKB: P23297
Human S100A1 Predicted Functions
Enables ATPase binding activity; S100 protein binding activity; and protein homodimerization activity. Involved in positive regulation of nitric-oxide synthase activity and positive regulation of sprouting angiogenesis. Located in several cellular components, including Golgi apparatus; nucleoplasm; and sarcoplasmic reticulum.
Mouse S100a1 Target Information
- Target Symbol: S100a1, S100 calcium binding protein A1
- Alias:
- AI266795
- expressed sequence AI266795
- S100
- S100a
- NCBI_Gene: 20193
Mouse S100a1 Predicted Functions
Predicted to enable several functions, including ATPase binding activity; S100 protein binding activity; and protein homodimerization activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II; positive regulation of nitric-oxide synthase activity; and positive regulation of sprouting angiogenesis. Located in sarcoplasmic reticulum. Is expressed in several structures, including brain; heart; inner ear; musculature; and urothelium. Orthologous to human S100A1 (S100 calcium binding protein A1).
Rat S100a1 Target Information
- Target Symbol: S100a1, S100 calcium binding protein A1
- Alias:
- protein S100-A1
- S-100 protein alpha chain
- S-100 protein subunit alpha
- S100 calcium-binding protein A1
- NCBI_Gene: 295214
Rat S100a1 Predicted Functions
Enables calcium ion binding activity; calcium-dependent protein binding activity; and identical protein binding activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of voltage-gated calcium channel activity. Located in M band and Z disc. Orthologous to human S100A1 (S100 calcium binding protein A1).
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