EK2SA1EG

EasyGo!™ Mouse S100A1 One-Step ELISA Kit

$349.00$499.00

 DATASHEET
Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100uL(diluent)
Sensitivity4.85 pg/ml
Array Range31.25-2000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
SKU: EK2SA1EG Category:

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100uL(diluent)
Sensitivity4.85 pg/ml
Array Range31.25-2000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
Intra Precision< 10%
Inter Precision< 10%
PlateDetachable 96-well Plate
Storage2-8℃
Deliveryblue ice
ComponentsCoated Microplate
standard
Assay Buffer (10×)
TMB
Stop Solution
Washing Buffer (20×)
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

S100A1

S100A1 Target Infomation Overview

  • Target Symbol: S100A1, S100 calcium binding protein A1
  • Gene Groups: S100 calcium binding proteins; EF-hand domain containing
  • Alias: S100-alpha, S100A
  • Alias Names: S100 calcium-binding protein A1

S100A1, epidermal growth factor receptor Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human S100A1 Target Information

  • Target Symbol: S100A1, S100 calcium binding protein A1
  • Alias:
    • protein S100-A1
    • S-100 protein alpha chain
    • S-100 protein subunit alpha
    • S100
    • S100 alpha
    • S100 calcium-binding protein A1
    • S100 protein, alpha polypeptide
    • S100-alpha
    • S100A
  • NCBI_Gene: 6271
  • UniProtKB: P23297

Human S100A1 Predicted Functions

Enables ATPase binding activity; S100 protein binding activity; and protein homodimerization activity. Involved in positive regulation of nitric-oxide synthase activity and positive regulation of sprouting angiogenesis. Located in several cellular components, including Golgi apparatus; nucleoplasm; and sarcoplasmic reticulum.

Mouse S100a1 Target Information

Mouse S100a1 Predicted Functions

Predicted to enable several functions, including ATPase binding activity; S100 protein binding activity; and protein homodimerization activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II; positive regulation of nitric-oxide synthase activity; and positive regulation of sprouting angiogenesis. Located in sarcoplasmic reticulum. Is expressed in several structures, including brain; heart; inner ear; musculature; and urothelium. Orthologous to human S100A1 (S100 calcium binding protein A1).

Rat S100a1 Target Information

  • Target Symbol: S100a1, S100 calcium binding protein A1
  • Alias:
    • protein S100-A1
    • S-100 protein alpha chain
    • S-100 protein subunit alpha
    • S100 calcium-binding protein A1
  • NCBI_Gene: 295214

Rat S100a1 Predicted Functions

Enables calcium ion binding activity; calcium-dependent protein binding activity; and identical protein binding activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of voltage-gated calcium channel activity. Located in M band and Z disc. Orthologous to human S100A1 (S100 calcium binding protein A1).

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