hCG ELISA Kit

$350.00$450.00

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SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity4.71 pg/mL
Range78.1 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery95% – 119%
Average Recovery1.08
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity4.71 pg/mL
Range78.1 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery95% – 119%
Average Recovery1.08
Intra Precision3.9% – 4.7%
Inter-Precision3.8%-4.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-hCG monoclonal antibody
Human hCG freeze-dried standard
hCG detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-hCG monoclonal antibodies are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, the hCG present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, horseradish peroxidase (HRP)-labeled streptavidin is added. After washing, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of hCG in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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