hCG ELISA Kit

$350.00$450.00

SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 80 μL 1×Assay Buffer and 20 μL sample
Sensitivity 4.71 pg/mL
Range 78.1 pg/mL – 5000 pg/mL
Assay Time 3.5 h
Recovery 95% – 119%
Average Recovery 1.08
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 80 μL 1×Assay Buffer and 20 μL sample
Sensitivity 4.71 pg/mL
Range 78.1 pg/mL – 5000 pg/mL
Assay Time 3.5 h
Recovery 95% – 119%
Average Recovery 1.08
Intra Precision 3.9% – 4.7%
Inter-Precision 3.8%-4.5%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-hCG monoclonal antibody
Human hCG freeze-dried standard
hCG detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-hCG monoclonal antibodies are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, the hCG present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, horseradish peroxidase (HRP)-labeled streptavidin is added. After washing, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of hCG in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Related Products

more +

EK1392

$350.00$450.00

EK1C01

$350.00$450.00

EK1242

$350.00$450.00

EK1341

$350.00$450.00

EK1334

$350.00$450.00

EK2295

$350.00$450.00

EK1360

$350.00$450.00

EK2100

$350.00$450.00

EK224

$350.00$450.00

EK1349

$350.00$450.00

EK397

$350.00$450.00

EK285

$350.00$450.00

EK2153

$350.00$450.00

EK387

$350.00$450.00

EK2F01

$350.00$450.00

EK2313

$350.00$450.00

Same Target