Human α-fetoprotein/AFP ELISA Kit

$350.00$450.00

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TARGETSAFP
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.45 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery90% – 110%
Average Recovery0.98
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.45 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery90% – 110%
Average Recovery0.98
Intra Precision1.6% – 2.2%
Inter-Precision1.6% – 3.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-α-fetoprotein/AFP monoclonal antibody
Human α-fetoprotein/AFP freeze-dried standard
α-fetoprotein/AFP detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human AFP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, AFP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of AFP in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

AFP

AFP Target Infomation Overview

  • Target Symbol: AFP, alpha fetoprotein
  • Alias: FETA
  • Previous Names: HPAFP
  • Alias Names: alpha-fetoprotein

AFP, alpha fetoprotein Target Infomation by Species

HumanMouseRat

Human AFP Target Information

Human AFP Predicted Functions

Predicted to enable fatty acid binding activity and zinc ion binding activity. Predicted to act upstream of or within SMAD protein signal transduction; ovulation from ovarian follicle; and progesterone metabolic process. Predicted to be located in endoplasmic reticulum lumen. Predicted to be active in cytoplasm. Implicated in hepatitis C. Biomarker of carcinoma (multiple); germ cell cancer (multiple); and reproductive organ cancer (multiple).

Mouse Afp Target Information

Mouse Afp Predicted Functions

Predicted to enable fatty acid binding activity and zinc ion binding activity. Acts upstream of or within SMAD protein signal transduction; ovulation from ovarian follicle; and progesterone metabolic process. Located in cytoplasm and extracellular space. Is expressed in several structures, including amniotic fluid; egg cylinder; gut; liver and biliary system; and metanephros. Human ortholog(s) of this gene implicated in hepatitis C. Orthologous to human AFP (alpha fetoprotein).

Rat Afp Target Information

Rat Afp Predicted Functions

Predicted to enable fatty acid binding activity and zinc ion binding activity. Involved in several processes, including cellular response to retinoic acid; liver regeneration; and response to dexamethasone. Located in cytosol and extracellular space. Biomarker of hepatitis. Human ortholog(s) of this gene implicated in hepatitis C. Orthologous to human AFP (alpha fetoprotein).

AFP Target News

    Transplantation
    Tumor Marker

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