Name: Human α-fetoprotein/AFP ELISA Kit
SKU: EK1175
Availability: InStock

EK1175

Human α-fetoprotein/AFP ELISA Kit

$245.00 – $350.00

Related Target	AFP
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.45 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	90% – 110%
Average Recovery	98%

ELISA Kit Detail Information

Related Target	AFP
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.45 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	90% – 110%
Average Recovery	98%
Intra Precision	1.6% – 2.2%
Inter Precision	1.6% – 3.3%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-α-fetoprotein/AFP monoclonal antibody Human α-fetoprotein/AFP freeze-dried standard α-fetoprotein/AFP detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human AFP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, AFP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of AFP in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

AFP

AFP Target Infomation Overview

Target Symbol: AFP, alpha fetoprotein
Alias: FETA
Previous Names: HPAFP
Alias Names: alpha-fetoprotein

AFP, alpha fetoprotein Target Infomation by Species

Human
Mouse
Rat

Human AFP Target Information

Target Symbol: AFP, alpha fetoprotein
Alias:
- AFPD
- alpha-1-fetoprotein
- alpha-fetoglobulin
- alpha-fetoprotein
- FETA
- HPAFP
NCBI_Gene: 174
UniProtKB: P02771

Human AFP Predicted Functions

Predicted to enable fatty acid binding activity and zinc ion binding activity. Predicted to act upstream of or within SMAD protein signal transduction; ovulation from ovarian follicle; and progesterone metabolic process. Predicted to be located in endoplasmic reticulum lumen. Predicted to be active in cytoplasm. Implicated in hepatitis C. Biomarker of carcinoma (multiple); germ cell cancer (multiple); and reproductive organ cancer (multiple).

Mouse Afp Target Information

Target Symbol: Afp, alpha fetoprotein
Alias:
- alpha-foetoprotein
NCBI_Gene: 11576

Mouse Afp Predicted Functions

Predicted to enable fatty acid binding activity and zinc ion binding activity. Acts upstream of or within SMAD protein signal transduction; ovulation from ovarian follicle; and progesterone metabolic process. Located in cytoplasm and extracellular space. Is expressed in several structures, including amniotic fluid; egg cylinder; gut; liver and biliary system; and metanephros. Human ortholog(s) of this gene implicated in hepatitis C. Orthologous to human AFP (alpha fetoprotein).

Rat Afp Target Information

Target Symbol: Afp, alpha-fetoprotein
Alias:
- alpha-1-fetoprotein
- alpha-fetoglobulin
- chloride intracellular channel 6
NCBI_Gene: 24177

Rat Afp Predicted Functions

Predicted to enable fatty acid binding activity and zinc ion binding activity. Involved in several processes, including cellular response to retinoic acid; liver regeneration; and response to dexamethasone. Located in cytosol and extracellular space. Biomarker of hepatitis. Human ortholog(s) of this gene implicated in hepatitis C. Orthologous to human AFP (alpha fetoprotein).

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