Human B7-1/CD80 ELISA Kit

$350.00$450.00

Download:
TARGETSCD80
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL 1×Assay Buffer and 50 μL sample
Sensitivity0.72 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery82% – 102%
Average Recovery0.91
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL 1×Assay Buffer and 50 μL sample
Sensitivity0.72 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery82% – 102%
Average Recovery0.91
Intra Precision3.7% – 5.0%
Inter-Precision2.8%-6.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-B7-1/CD80 monoclonal antibody
Human B7-1/CD80 freeze-dried standard
B7-1/CD80 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CD80 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CD80 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD80 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 


Warning: Undefined array key 1 in /www/wwwroot/test.liankebio.cn/wp-includes/media.php on line 71

Targets

CD80

CD80 Target Infomation Overview

  • Target Symbol: CD80, CD80 molecule
  • Gene Groups: CD molecules; V-set domain containing; C2-set domain containing; B7 family
  • Alias: B7.1; B7-1
  • Previous Names: CD28LG; CD28LG1
  • Alias Names: B-lymphocyte activation antigen B7; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule

CD80, CD80 molecule Target Infomation by Species

HumanMouseRat

Human CD80 Target Information

  • Target Symbol: CD80, CD80 molecule
  • Alias:
    • activation B7-1 antigen
    • B-lymphocyte activation antigen B7
    • B7
    • B7-1
    • B7.1
    • BB1
    • CD28LG
    • CD28LG1
    • cd80 antigen
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
    • costimulatory factor CD80
    • costimulatory molecule variant IgV-CD80
    • CTLA-4 counter-receptor B7.1
    • LAB7
    • T-lymphocyte activation antigen CD80
  • NCBI_Gene: 941
  • UniProtKB: P33681

Human CD80 Predicted Functions

Predicted to enable virus receptor activity. Involved in positive regulation of peptidyl-tyrosine phosphorylation. Located in cell surface. Part of protein complex involved in cell adhesion. Implicated in type 1 diabetes mellitus. Biomarker of chronic obstructive pulmonary disease; demyelinating disease; relapsing-remitting multiple sclerosis; rheumatoid arthritis; and systemic lupus erythematosus.

Mouse Cd80 Target Information

Mouse Cd80 Predicted Functions

Predicted to enable coreceptor activity. Acts upstream of or within T cell costimulation; cellular response to lipopolysaccharide; and positive regulation of alpha-beta T cell proliferation. Located in external side of plasma membrane. Is expressed in heart ventricle and skin. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human CD80 (CD80 molecule).

Rat Cd80 Target Information

Rat Cd80 Predicted Functions

Predicted to enable coreceptor activity. Involved in positive regulation of T cell proliferation. Located in cell surface. Used to study adult T-cell leukemia; anti-basement membrane glomerulonephritis; high grade glioma; and periodontal disease. Biomarker of kidney failure. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human CD80 (CD80 molecule).

CD80 Target News

Multiple Sclerosis

Related Products

more +

EK1392

$350.00$450.00

EK1341

$350.00$450.00

EK1C01

$350.00$450.00

Same Target