Product Category
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Human B7-1/CD80 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL 1×Assay Buffer and 50 μL sample |
| Sensitivity | 0.72 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 82% – 102% |
| Average Recovery | 0.91 |
| Intra Precision | 3.7% – 5.0% |
| Inter-Precision | 2.8%-6.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-B7-1/CD80 monoclonal antibody Human B7-1/CD80 freeze-dried standard B7-1/CD80 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CD80 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CD80 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD80 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
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Targets
CD80
CD80 Target Infomation Overview
- Target Symbol: CD80, CD80 molecule
- Gene Groups: CD molecules; V-set domain containing; C2-set domain containing; B7 family
- Alias: B7.1; B7-1
- Previous Names: CD28LG; CD28LG1
- Alias Names: B-lymphocyte activation antigen B7; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule
CD80, CD80 molecule Target Infomation by Species
HumanMouseRat
Human CD80 Target Information
- Target Symbol: CD80, CD80 molecule
- Alias:
- activation B7-1 antigen
- B-lymphocyte activation antigen B7
- B7
- B7-1
- B7.1
- BB1
- CD28LG
- CD28LG1
- cd80 antigen
- CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
- costimulatory factor CD80
- costimulatory molecule variant IgV-CD80
- CTLA-4 counter-receptor B7.1
- LAB7
- T-lymphocyte activation antigen CD80
- NCBI_Gene: 941
- UniProtKB: P33681
Human CD80 Predicted Functions
Predicted to enable virus receptor activity. Involved in positive regulation of peptidyl-tyrosine phosphorylation. Located in cell surface. Part of protein complex involved in cell adhesion. Implicated in type 1 diabetes mellitus. Biomarker of chronic obstructive pulmonary disease; demyelinating disease; relapsing-remitting multiple sclerosis; rheumatoid arthritis; and systemic lupus erythematosus.
Mouse Cd80 Target Information
- Target Symbol: Cd80, CD80 antigen
- Alias:
- B7-1
- B7.1
- CD28 antigen ligand
- Cd28l
- Ly-53
- Ly53
- lymphocyte antigen 53
- NCBI_Gene: 12519
Mouse Cd80 Predicted Functions
Predicted to enable coreceptor activity. Acts upstream of or within T cell costimulation; cellular response to lipopolysaccharide; and positive regulation of alpha-beta T cell proliferation. Located in external side of plasma membrane. Is expressed in heart ventricle and skin. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human CD80 (CD80 molecule).
Rat Cd80 Target Information
- Target Symbol: Cd80, Cd80 molecule
- Alias:
- B7-1
- Cd80 a rat homolog of the human CD28/CTLA – 4 ligand (B7-1)
- CD80 antigen
- CD80-like
- LOC100360171
- T-lymphocyte activation antigen CD80
- NCBI_Gene: 25408
Rat Cd80 Predicted Functions
Predicted to enable coreceptor activity. Involved in positive regulation of T cell proliferation. Located in cell surface. Used to study adult T-cell leukemia; anti-basement membrane glomerulonephritis; high grade glioma; and periodontal disease. Biomarker of kidney failure. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human CD80 (CD80 molecule).
