Name: Human Complement Factor H/CFH ELISA Kit
SKU: EK1305
Availability: InStock

EK1305

Human Complement Factor H/CFH ELISA Kit

$245.00 – $350.00

Related Target	CFH
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	16.57 pg/mL
Array Range	0.78 ng/mL – 50 ng/mL
Assay Time	3.5 h
Recovery	90% – 108%
Average Recovery	95%

ELISA Kit Detail Information

Related Target	CFH
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	16.57 pg/mL
Array Range	0.78 ng/mL – 50 ng/mL
Assay Time	3.5 h
Recovery	90% – 108%
Average Recovery	95%
Intra Precision	3.1% – 6.3%
Inter Precision	4.5% - 5.6%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CFH monoclonal antibody Human CFH freeze-dried standard Human CFH detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CFH antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CFH present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CFH in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CFH

CFH Target Infomation Overview

Target Symbol: CFH, complement factor H
Gene Groups: Sushi domain containing; Complement system regulators and receptors
Alias: HUS; FHL1; ARMS1; ARMD4
Previous Names: HF; HF1; HF2
Alias Names: beta-1H; H factor 2 (complement); age-related maculopathy susceptibility 1; H factor 1 (complement)

CFH, complement factor H Target Infomation by Species

Human
Mouse
Rat

Human CFH Target Information

Target Symbol: CFH, complement factor H
Alias:
- adrenomedullin binding protein
- age-related maculopathy susceptibility 1
- AHUS1
- AMBP1
- ARMD4
- ARMS1
- beta-1-H-globulin
- beta-1H
- CFHL3
- complement factor H, isoform b
- factor H
- factor H-like 1
- FH
- FHL1
- H factor 1 (complement)
- H factor 2 (complement)
- HF
- HF1
- HF2
- HUS
- MGC88246
NCBI_Gene: 3075
UniProtKB: P08603

Human CFH Predicted Functions

Enables heparan sulfate proteoglycan binding activity; heparin binding activity; and identical protein binding activity. Involved in complement activation. Acts upstream of or within regulation of complement activation and regulation of complement-dependent cytotoxicity. Located in blood microparticle and extracellular exosome. Implicated in several diseases, including atypical hemolytic-uremic syndrome; eye disease (multiple); hemolytic-uremic syndrome; lupus nephritis; and systemic lupus erythematosus. Biomarker of amphetamine abuse; atypical hemolytic-uremic syndrome; glaucoma; psoriasis; and squamous cell carcinoma.

Mouse Cfh Target Information

Target Symbol: Cfh, complement component factor h
Alias:
- Mud-1
- Sas-1
- Sas1
- serum antigenic substance 1
NCBI_Gene: 12628

Mouse Cfh Predicted Functions

Enables complement component C3b binding activity and heparin binding activity. Acts upstream of or within several processes, including animal organ development; complement activation; and vasculature development. Located in several cellular components, including axon; external side of plasma membrane; and neuronal cell body. Is expressed in several structures, including adrenal gland; bone; central nervous system; humerus cartilage condensation; and metanephros. Used to study age related macular degeneration 4; atypical hemolytic-uremic syndrome; hemolytic-uremic syndrome; and membranoproliferative glomerulonephritis. Human ortholog(s) of this gene implicated in several diseases, including atypical hemolytic-uremic syndrome; eye disease (multiple); hemolytic-uremic syndrome; lupus nephritis; and systemic lupus erythematosus. Orthologous to several human genes including CFH (complement factor H).

Rat Cfh Target Information

Target Symbol: Cfh, complement factor H
Alias:
- adrenomedullin binding protein-1
- AMBP-1
- AMBP1
- complement component factor H
- complement inhibitory factor H
- Fh
- platelet complement factor H
NCBI_Gene: 155012

Rat Cfh Predicted Functions

Predicted to enable several functions, including complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Involved in several processes, including cellular response to hydrogen peroxide; cellular response to interferon-gamma; and cellular response to lipopolysaccharide. Located in several cellular components, including extracellular space; nucleus; and plasma membrane. Used to study atypical hemolytic-uremic syndrome. Biomarker of amphetamine abuse; membranous glomerulonephritis; obstructive jaundice; proteinuria (multiple); and retinal degeneration. Human ortholog(s) of this gene implicated in several diseases, including atypical hemolytic-uremic syndrome; eye disease (multiple); hemolytic-uremic syndrome; lupus nephritis; and systemic lupus erythematosus. Orthologous to several human genes including CFH (complement factor H).

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