Human CX3CL1/Fractalkine ELISA Kit

$350.00$450.00

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TARGETSCX3CL1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity34.45 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery80% – 102%
Average Recovery0.88
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity34.45 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery80% – 102%
Average Recovery0.88
Intra Precision2.1% – 3.6%
Inter-Precision2.6% – 4.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CX3CL1 monoclonal antibody
Human CX3CL1 freeze-dried standard
CX3CL1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CX3CL1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CX3CL1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of CX3CL1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CX3CL1

CX3CL1 Target Infomation Overview

  • Target Symbol: CX3CL1, C-X3-C motif chemokine ligand 1
  • Gene Groups: Chemokine ligands
  • Alias: NTN; C3Xkine; ABCD-3; CXC3C; CXC3
  • Previous Names: SCYD1
  • Alias Names: fractalkine; neurotactin; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); chemokine (C-X3-C motif) ligand 1

CX3CL1, C-X3-C motif chemokine ligand 1 Target Infomation by Species

HumanMouseRat

Human CX3CL1 Target Information

  • Target Symbol: CX3CL1, C-X3-C motif chemokine ligand 1
  • Alias:
    • ABCD-3
    • C-X3-C motif chemokine 1
    • C3Xkine
    • chemokine (C-X3-C motif) ligand 1
    • CX3C membrane-anchored chemokine
    • CXC3
    • CXC3C
    • fractalkine
    • neurotactin
    • NTN
    • NTT
    • SCYD1
    • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
    • small inducible cytokine subfamily D (Cys-X3-Cys), member-1
    • small-inducible cytokine D1
  • NCBI_Gene: 6376
  • UniProtKB: P78423

Human CX3CL1 Predicted Functions

Enables signaling receptor binding activity. Involved in several processes, including regulation of cell migration; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular region; and membrane. Is integral component of membrane. Implicated in Cor pulmonale and common cold. Biomarker of several diseases, including chronic fatigue syndrome; myositis (multiple); polyarteritis nodosa; pulmonary hypertension; and rheumatoid arthritis.

Mouse Cx3cl1 Target Information

Mouse Cx3cl1 Predicted Functions

Enables CX3C chemokine receptor binding activity and chemokine activity. Acts upstream of or within several processes, including angiogenesis involved in wound healing; leukocyte chemotaxis; and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand. Located in several cellular components, including cell surface; neuron projection; and neuronal cell body. Is expressed in several structures, including brain; genitourinary system; gut; nose; and respiratory system. Human ortholog(s) of this gene implicated in Cor pulmonale and common cold. Orthologous to human CX3CL1 (C-X3-C motif chemokine ligand 1).

Rat Cx3cl1 Target Information

  • Target Symbol: Cx3cl1, C-X3-C motif chemokine ligand 1
  • Alias:
    • C-X3-C motif chemokine 1
    • chemokine (C-X3-C motif) ligand 1
    • Cx3c
    • CX3C membrane-anchored chemokine
    • fractalkine
    • neurotactin
    • Scyd1
    • small inducible cytokine subfamily D 1
    • small inducible cytokine subfamily D, 1
    • small-inducible cytokine D1
  • NCBI_Gene: 89808

Rat Cx3cl1 Predicted Functions

Enables chemokine activity. Involved in several processes, including positive regulation of cell population proliferation; regulation of gene expression; and regulation of signal transduction. Located in several cellular components, including neuron projection; neuronal cell body; and perinuclear region of cytoplasm. Used to study acute pancreatitis; ischemia; and status epilepticus. Biomarker of several diseases, including borna disease; brain disease (multiple); liver disease (multiple); lung disease (multiple); and pulmonary hypertension. Human ortholog(s) of this gene implicated in Cor pulmonale and common cold. Orthologous to human CX3CL1 (C-X3-C motif chemokine ligand 1).

CX3CL1 Target News

    11.3
    Blockage of CacyBP inhibits macrophage recruitment and improves anti-PD-1 therapy in hepatocellular carcinoma
    JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Osteoarthrosis & Rheumatoid Arthritis

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