Name: Human CXCL13/BLC/BCA-1 ELISA Kit
SKU: EK1105
Availability: InStock

EK1105

Human CXCL13/BLC/BCA-1 ELISA Kit

$245.00 – $350.00

Related Target	CXCL13
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	4.86 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	87% – 114%
Average Recovery	99%

ELISA Kit Detail Information

Related Target	CXCL13
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	4.86 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	87% – 114%
Average Recovery	99%
Intra Precision	1.3% – 6.5%
Inter Precision	2.7% – 4.9%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL13/BLC/BCA-1 monoclonal antibody Human CXCL13/BLC/BCA-1 freeze-dried standard CXCL13/BLC/BCA-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL13 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CXCL13 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL13 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CXCL13

CXCL13 Target Infomation Overview

Target Symbol: CXCL13, C-X-C motif chemokine ligand 13
Gene Groups: Chemokine ligands
Alias: BLC; BCA-1; BLR1L; ANGIE; ANGIE2
Previous Names: SCYB13
Alias Names: B-cell chemoattractant; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13

CXCL13, C-X-C motif chemokine ligand 13 Target Infomation by Species

Human
Mouse
Rat

Human CXCL13 Target Information

Target Symbol: CXCL13, C-X-C motif chemokine ligand 13
Alias:
- ANGIE
- ANGIE2
- b cell-attracting chemokine 1
- b lymphocyte chemoattractant
- B-cell chemoattractant
- B-cell-attracting chemokine 1
- B-cell-homing chemokine (ligand for Burkitt’s lymphoma receptor-1)
- B-lymphocyte chemoattractant
- BCA-1
- BCA1
- BLC
- BLR1L
- C-X-C motif chemokine 13
- chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
- CXC chemokine BLC
- SCYB13
- small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
- small-inducible cytokine B13
NCBI_Gene: 10563
UniProtKB: O43927

Human CXCL13 Predicted Functions

Enables chemokine receptor binding activity; fibroblast growth factor binding activity; and heparin binding activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; cell chemotaxis; and regulation of chemotaxis. Located in extracellular region.

Mouse Cxcl13 Target Information

Target Symbol: Cxcl13, chemokine (C-X-C motif) ligand 13
Alias:
- 4631412M08Rik
- ANGIE2
- BCA-1
- BLC
- RIKEN cDNA 4631412M08 gene
- Scyb13
- small inducible cytokine subfamily B (Cys-X-Cys), member 13
NCBI_Gene: 55985

Mouse Cxcl13 Predicted Functions

Enables CXCR5 chemokine receptor binding activity and chemokine activity. Involved in several processes, including B cell chemotaxis across high endothelial venule; activation of GTPase activity; and lymph node development. Located in extracellular space. Is expressed in Peyer’s patch; aorta; central nervous system; and retina. Orthologous to human CXCL13 (C-X-C motif chemokine ligand 13).

Rat Cxcl13 Target Information

Target Symbol: Cxcl13, C-X-C motif chemokine ligand 13
Alias:
- C-X-C motif chemokine 13
- chemokine (C-X-C motif) ligand 13
- LOC498335
- similar to Small inducible cytokine B13 precursor (CXCL13) (B lymphocyte chemoattractant) (CXC chemokine BLC)
NCBI_Gene: 498335
- UniprotKB: F7F7W7
- UniprotKB: Q5I0J6

Rat Cxcl13 Predicted Functions

Predicted to enable chemokine receptor binding activity; fibroblast growth factor binding activity; and heparin binding activity. Predicted to be involved in several processes, including cell chemotaxis; regulation of chemotaxis; and response to bacterium. Predicted to act upstream of or within lymph node development. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Orthologous to human CXCL13 (C-X-C motif chemokine ligand 13).

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