Human CXCL3/GRO-γ ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CXCL3
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	0.24 pg/mL
Range	1.56 pg/mL – 100 pg/mL
Assay Time	3.5 h
Recovery	86% – 105%
Average Recovery	0.95

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	0.24 pg/mL
Range	1.56 pg/mL – 100 pg/mL
Assay Time	3.5 h
Recovery	86% – 105%
Average Recovery	0.95
Intra Precision	2.6% – 5.5%
Inter-Precision	2.3% – 11.5%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL3/GRO-γ monoclonal antibody Human CXCL3/GRO-γ freeze-dried standard Angiotensinogen/AGT/ SerpinA8 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL3 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the CXCL3 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CXCL3

CXCL3 Target Infomation Overview

Target Symbol: CXCL3, C-X-C motif chemokine ligand 3
Gene Groups: Chemokine ligands
Alias: SCYB3; GROg; MIP-2b; CINC-2b
Previous Names: GRO3
Alias Names: GRO3 oncogene; chemokine (C-X-C motif) ligand 3

CXCL3, C-X-C motif chemokine ligand 3 Target Infomation by Species

HumanMouseRat

Human CXCL3 Target Information

Target Symbol: CXCL3, C-X-C motif chemokine ligand 3
Alias:
- C-X-C motif chemokine 3
- chemokine (C-X-C motif) ligand 3
- CINC-2b
- GRO-gamma
- GRO-gamma(1-73)
- GRO3
- GRO3 oncogene
- GROg
- growth-regulated protein gamma
- macrophage inflammatory protein 2-beta
- melanoma growth stimulatory activity gamma
- MGSA gamma
- MIP-2b
- MIP2-beta
- MIP2B
- SCYB3
NCBI_Gene: 2921
UniProtKB: P19876

Human CXCL3 Predicted Functions

Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide and killing of cells of other organism. Located in extracellular space.

Mouse Cxcl3 Target Information

Target Symbol: Cxcl3, chemokine (C-X-C motif) ligand 3
Alias:
- Dcip1
- gene model 1960, (NCBI)
- Gm1960
NCBI_Gene: 330122
- UniprotKB: Q6W5C0

Mouse Cxcl3 Predicted Functions

Enables chemokine activity. Involved in neutrophil chemotaxis. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in genitourinary system; gut; and thymus. Orthologous to human CXCL1 (C-X-C motif chemokine ligand 1); CXCL2 (C-X-C motif chemokine ligand 2); and CXCL3 (C-X-C motif chemokine ligand 3).

Rat Cxcl3 Target Information

Target Symbol: Cxcl3, C-X-C motif chemokine ligand 3
Alias:
- C-X-C motif chemokine 3
- chemokine (C-X-C motif) ligand 3
- Cinc-2
- Cinc2
- cytokine-induced neutrophil chemoattractant 2
- cytokine-induced neutrophil chemoattractant-2
- gene model 1960, (NCBI)
- Gm1960
- macrophage inflammatory protein 2-alpha/beta
- MIP2-alpha/beta
NCBI_Gene: 171551
UniProtKB: Q10746

Rat Cxcl3 Predicted Functions

Enables chemokine activity. Involved in several processes, including cellular response to lipopolysaccharide; neutrophil chemotaxis; and positive regulation of cytosolic calcium ion concentration. Located in extracellular space. Biomarker of Coronavirus infectious disease and bacterial pneumonia. Orthologous to human CXCL1 (C-X-C motif chemokine ligand 1); CXCL2 (C-X-C motif chemokine ligand 2); and CXCL3 (C-X-C motif chemokine ligand 3).