Human CXCL9/MIG ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 0.06 pg/mL |
| Array Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 120% |
| Average Recovery | 104% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 0.06 pg/mL |
| Array Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 120% |
| Average Recovery | 104% |
| Intra Precision | 2.6% – 3.4% |
| Inter Precision | 2.4% – 3.0% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL9/MIG monoclonal antibody Human CXCL9/MIG freeze-dried standard CXCL9/MIG detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CXCL9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL9 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
CXCL9
CXCL9 Target Infomation Overview
- Target Symbol: CXCL9, C-X-C motif chemokine ligand 9
- Gene Groups: Chemokine ligands
- Alias: SCYB9; Humig; crg-10
- Previous Names: CMK; MIG
- Alias Names: monokine induced by gamma interferon; chemokine (C-X-C motif) ligand 9
CXCL9, C-X-C motif chemokine ligand 9 Target Infomation by Species
- Human
- Mouse
- Rat
Human CXCL9 Target Information
- Target Symbol: CXCL9, C-X-C motif chemokine ligand 9
- Alias:
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
- CMK
- crg-10
- gamma-interferon-induced monokine
- Humig
- MIG
- monokine induced by gamma interferon
- monokine induced by interferon-gamma
- SCYB9
- small-inducible cytokine B9
- NCBI_Gene: 4283
- UniProtKB: Q07325
Human CXCL9 Predicted Functions
Enables CXCR3 chemokine receptor binding activity and chemokine activity. Involved in several processes, including adenylate cyclase-activating G protein-coupled receptor signaling pathway; antimicrobial humoral immune response mediated by antimicrobial peptide; and positive regulation of release of sequestered calcium ion into cytosol. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including chronic fatigue syndrome; cystic fibrosis; lung disease (multiple); severe acute respiratory syndrome; and thoracic aortic aneurysm.
Mouse Cxcl9 Target Information
- Target Symbol: Cxcl9, chemokine (C-X-C motif) ligand 9
- Alias:
- BB139920
- CMK
- crg-10
- expressed sequence BB139920
- Mig
- monokine induced by gamma interferon
- Scyb9
- small inducible cytokine B subfamily (Cys-X-Cys), member 9
- NCBI_Gene: 17329
Mouse Cxcl9 Predicted Functions
Predicted to enable CXCR3 chemokine receptor binding activity and chemokine activity. Acts upstream of or within several processes, including defense response to virus; positive regulation of myoblast differentiation; and positive regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in central nervous system; retina; stomach; testis; and thymus. Orthologous to human CXCL9 (C-X-C motif chemokine ligand 9).
Rat Cxcl9 Target Information
- Target Symbol: Cxcl9, C-X-C motif chemokine ligand 9
- Alias:
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
- MGC105312
- Mig
- monokine induced by gamma interferon
- Scyb9
- small inducible cytokine B9
- NCBI_Gene: 246759
- UniProtKB: Q8K4B1
Rat Cxcl9 Predicted Functions
Predicted to enable CXCR3 chemokine receptor binding activity and chemokine activity. Predicted to be involved in several processes, including cellular response to lipopolysaccharide; neutrophil chemotaxis; and positive regulation of release of sequestered calcium ion into cytosol. Predicted to act upstream of or within several processes, including defense response to virus; positive regulation of myoblast differentiation; and positive regulation of myoblast fusion. Predicted to be located in external side of plasma membrane. Predicted to be active in extracellular space. Orthologous to human CXCL9 (C-X-C motif chemokine ligand 9).
Products with the Same Target - CXCL9
Citations for Human CXCL9/MIG ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK1258
- ELISA Kit
Human Apolipoprotein AII/Apo A2 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1299
- ELISA Kit
hCG ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1109
- ELISA Kit
Human sTNF RI/TNFRSF1A ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1204
- ELISA Kit
Human Apolipoprotein A1/Apo A1 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1260
- ELISA Kit
Human alpha-Galactosidase A/GLA ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1263
- ELISA Kit
Human 4-1BB/TNFRSF9/CD137 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.