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Human Decorin ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL(diluent) |
| Sensitivity | 0.083 pg/mL |
| Range | 3.13 pg/mL – 200 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 79% – 116% |
| Average Recovery | 0.91 |
| Intra Precision | 3.3% – 5.0% |
| Inter-Precision | 1.7%-3.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Decorin monoclonal antibody Human Decorin freeze-dried standard Decorin detect Antibody Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Decorin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Decorin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Decorin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
DCN
DCN Target Infomation Overview
- Target Symbol: DCN, decorin
- Gene Groups: Small leucine rich repeat proteoglycans
- Alias: DSPG2; SLRR1B
- Alias Names: decorin proteoglycan
DCN, decorin Target Infomation by Species
HumanMouseRat
Human DCN Target Information
- Target Symbol: DCN, decorin
- Alias:
- bone proteoglycan II
- CSCD
- decorin proteoglycan
- dermatan sulphate proteoglycans II
- DSPG2
- PG-S2
- PG40
- PGII
- PGS2
- proteoglycan core protein
- SLRR1B
- small leucine-rich protein 1B
- NCBI_Gene: 1634
- UniProtKB: P07585
Human DCN Predicted Functions
Enables RNA binding activity. Involved in positive regulation of autophagy. Acts upstream of or within several processes, including positive regulation of mitochondrial depolarization; positive regulation of mitochondrial fission; and regulation of signal transduction. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in breast carcinoma; congenital stromal corneal dystrophy; and high grade glioma. Biomarker of gestational diabetes and type 2 diabetes mellitus.
Mouse Dcn Target Information
- Target Symbol: Dcn, decorin
- Alias:
- DC
- SLRR1B
- NCBI_Gene: 13179
Mouse Dcn Predicted Functions
Enables extracellular matrix binding activity and glycosaminoglycan binding activity. Acts upstream of or within peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan. Located in collagen-containing extracellular matrix. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; genitourinary system; and sensory organ. Used to study Ehlers-Danlos syndrome and congenital stromal corneal dystrophy. Human ortholog(s) of this gene implicated in breast carcinoma; congenital stromal corneal dystrophy; and high grade glioma. Orthologous to human DCN (decorin).
Rat Dcn Target Information
- Target Symbol: Dcn, decorin
- Alias:
- bone proteoglycan II
- dermatan sulfate proteoglycan-II (decorin)
- DSPG
- MGC94682
- PG-S2
- PG40
- NCBI_Gene: 29139
- UniProtKB: Q01129
Rat Dcn Predicted Functions
Enables collagen binding activity and protein N-terminus binding activity. Involved in several processes, including animal organ development; response to lipopolysaccharide; and wound healing. Located in extracellular matrix. Part of collagen type VI trimer. Biomarker of asthma; myocardial infarction; and obesity. Human ortholog(s) of this gene implicated in breast carcinoma; congenital stromal corneal dystrophy; and high grade glioma. Orthologous to human DCN (decorin).
