Human Decorin ELISA Kit

$245.00 – $350.00

Related Target	DCN
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.083 pg/mL
Array Range	3.13 pg/mL – 200 pg/mL
Assay Time	3.5 h
Recovery	79% – 116%
Average Recovery	91%

ELISA Kit Detail Information

Related Target	DCN
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.083 pg/mL
Array Range	3.13 pg/mL – 200 pg/mL
Assay Time	3.5 h
Recovery	79% – 116%
Average Recovery	91%
Intra Precision	3.3% – 5.0%
Inter Precision	1.7% - 3.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Decorin monoclonal antibody Human Decorin freeze-dried standard Decorin detect Antibody Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Decorin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Decorin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Decorin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

DCN

DCN Target Infomation Overview

Target Symbol: DCN, decorin
Gene Groups: Small leucine rich repeat proteoglycans
Alias: DSPG2; SLRR1B
Alias Names: decorin proteoglycan

DCN, decorin Target Infomation by Species

Human
Mouse
Rat

Human DCN Target Information

Target Symbol: DCN, decorin
Alias:
- bone proteoglycan II
- CSCD
- decorin proteoglycan
- dermatan sulphate proteoglycans II
- DSPG2
- PG-S2
- PG40
- PGII
- PGS2
- proteoglycan core protein
- SLRR1B
- small leucine-rich protein 1B
NCBI_Gene: 1634
UniProtKB: P07585

Human DCN Predicted Functions

Enables RNA binding activity. Involved in positive regulation of autophagy. Acts upstream of or within several processes, including positive regulation of mitochondrial depolarization; positive regulation of mitochondrial fission; and regulation of signal transduction. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in breast carcinoma; congenital stromal corneal dystrophy; and high grade glioma. Biomarker of gestational diabetes and type 2 diabetes mellitus.

Mouse Dcn Target Information

Mouse Dcn Predicted Functions

Enables extracellular matrix binding activity and glycosaminoglycan binding activity. Acts upstream of or within peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan. Located in collagen-containing extracellular matrix. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; genitourinary system; and sensory organ. Used to study Ehlers-Danlos syndrome and congenital stromal corneal dystrophy. Human ortholog(s) of this gene implicated in breast carcinoma; congenital stromal corneal dystrophy; and high grade glioma. Orthologous to human DCN (decorin).

Rat Dcn Target Information

Target Symbol: Dcn, decorin
Alias:
- bone proteoglycan II
- dermatan sulfate proteoglycan-II (decorin)
- DSPG
- MGC94682
- PG-S2
- PG40
NCBI_Gene: 29139
UniProtKB: Q01129

Rat Dcn Predicted Functions

Enables collagen binding activity and protein N-terminus binding activity. Involved in several processes, including animal organ development; response to lipopolysaccharide; and wound healing. Located in extracellular matrix. Part of collagen type VI trimer. Biomarker of asthma; myocardial infarction; and obesity. Human ortholog(s) of this gene implicated in breast carcinoma; congenital stromal corneal dystrophy; and high grade glioma. Orthologous to human DCN (decorin).

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