Name: Human Endothelin 1/ET-1 Competitive ELISA Kit
SKU: EK1314
Availability: InStock

EK1314

Human Endothelin 1/ET-1 Competitive ELISA Kit

$245.00 – $350.00

Related Target	EDN1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (original)
Sensitivity	0.11 pg/mL
Array Range	0.16 pg/mL – 20 pg/mL
Assay Time	3.5 h
Recovery	90% – 108%
Average Recovery	98%

Size

Clear

ELISA Kit Detail Information

Related Target	EDN1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (original)
Sensitivity	0.11 pg/mL
Array Range	0.16 pg/mL – 20 pg/mL
Assay Time	3.5 h
Recovery	90% – 108%
Average Recovery	98%
Intra Precision	3.1% – 6.2%
Inter Precision	4.2% - 7.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ET-1 monoclonal antibody Human ET-1 freeze-dried standard Angiotensinogen/AGT/ SerpinA8 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes a solid-phase competitive enzyme-linked immunosorbent assay (ELISA) technique. Specific monoclonal antibodies against human ET-1 are precoated on a high-affinity ELISA plate. Biotinylated ET-1 and unlabeled ET-1 or samples are added into the wells of the ELISA plate, competing to bind to the limited binding sites on the coated antibody. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is inversely proportional to the concentration of ET-1 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

EDN1

EDN1 Target Infomation Overview

Target Symbol: EDN1, endothelin 1
Gene Groups: Endothelins
Alias: ET1

EDN1, endothelin 1 Target Infomation by Species

Human
Mouse
Rat

Human EDN1 Target Information

Target Symbol: EDN1, endothelin 1
Alias:
- ARCND3
- endothelin-1
- ET1
- HDLCQ7
- PPET1
- preproendothelin-1
- QME
NCBI_Gene: 1906
UniProtKB: P05305

Human EDN1 Predicted Functions

Enables signaling receptor binding activity. Involved in several processes, including intracellular signal transduction; regulation of macromolecule biosynthetic process; and vascular associated smooth muscle contraction. Located in cytoplasm and extracellular space. Implicated in several diseases, including artery disease (multiple); bronchial disease (multiple); obesity; obstructive sleep apnea; and rheumatoid arthritis. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); glaucoma (multiple); lung disease (multiple); and retinal disease (multiple).

Mouse Edn1 Target Information

Target Symbol: Edn1, endothelin 1
Alias:
- ET-1
NCBI_Gene: 13614
- UniprotKB: P22387
- UniprotKB: Q544E0

Mouse Edn1 Predicted Functions

Enables endothelin A receptor binding activity. Involved in several processes, including cellular response to gonadotropin stimulus; circulatory system development; and neuron differentiation. Acts upstream of or within several processes, including G protein-coupled receptor signaling pathway; animal organ development; and rhythmic excitation. Located in extracellular space. Is expressed in several structures, including branchial arch; cardiovascular system; central nervous system; gut; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); lung disease (multiple); obesity; obstructive sleep apnea; and rheumatoid arthritis. Orthologous to human EDN1 (endothelin 1).

Rat Edn1 Target Information

Target Symbol: Edn1, endothelin 1
Alias:
- dickkopf 1 homolog
- dickkopf 1 homolog (Xenopus laevis)
- dickkopf homolog 1
- dickkopf homolog 1 (Xenopus laevis)
- dickkopf-like protein 1
- dickkopf-related protein 1
- LOC293897
- NEWGENE_1307313
NCBI_Gene: 24323
UniProtKB: P22388

Rat Edn1 Predicted Functions

Predicted to enable co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in negative regulation of ossification and positive regulation of neuron death. Located in extracellular space. Used to study visual epilepsy. Human ortholog(s) of this gene implicated in anodontia. Orthologous to human DKK1 (dickkopf WNT signaling pathway inhibitor 1).

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