Human ErbB2/HER2/CD340 ELISA Kit

$350.00$450.00

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TARGETSERBB2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.51 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery85% – 112%
Average Recovery1.06
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.51 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery85% – 112%
Average Recovery1.06
Intra Precision1.9% – 3.2%
Inter-Precision2.1% – 3.7%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ErbB2/HER2/CD340 monoclonal antibody
Human ErbB2/HER2/CD340 freeze-dried standard
ErbB2/HER2/CD340 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ErbB2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, ErbB2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ErbB2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

ERBB2

ERBB2 Target Infomation Overview

  • Target Symbol: ERBB2, erb-b2 receptor tyrosine kinase 2
  • Gene Groups: CD molecules; Minor histocompatibility antigens; Erb-b2 receptor tyrosine kinases; MicroRNA protein coding host genes
  • Alias: NEU; HER-2; CD340; HER2
  • Previous Names: NGL
  • Alias Names: neuro/glioblastoma derived oncogene homolog; human epidermal growth factor receptor 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2

ERBB2, erb-b2 receptor tyrosine kinase 2 Target Infomation by Species

HumanMouseRat

Human ERBB2 Target Information

  • Target Symbol: ERBB2, erb-b2 receptor tyrosine kinase 2
  • Alias:
    • c-erb B2/neu protein
    • CD340
    • HER-2
    • HER-2/neu
    • HER2
    • herstatin
    • human epidermal growth factor receptor 2
    • metastatic lymph node gene 19 protein
    • MLN 19
    • NEU
    • neuro/glioblastoma derived oncogene homolog
    • neuroblastoma/glioblastoma derived oncogene homolog
    • NGL
    • p185erbB2
    • proto-oncogene c-ErbB-2
    • proto-oncogene Neu
    • receptor tyrosine-protein kinase erbB-2
    • TKR1
    • tyrosine kinase-type cell surface receptor HER2
    • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
    • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
    • v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2
    • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
    • VSCN2
  • NCBI_Gene: 2064
  • UniProtKB: P04626

Human ERBB2 Predicted Functions

Enables several functions, including ErbB-3 class receptor binding activity; enzyme binding activity; and protein C-terminus binding activity. Contributes to growth factor binding activity. Involved in several processes, including positive regulation of macromolecule biosynthetic process; protein phosphorylation; and regulation of MAPK cascade. Acts upstream of or within cell surface receptor signaling pathway. Located in several cellular components, including basolateral plasma membrane; cytosol; and endosome membrane. Part of ERBB3:ERBB2 complex. Implicated in carcinoma (multiple); high grade glioma; reproductive organ cancer (multiple); and urinary bladder cancer. Biomarker of several diseases, including carcinoma (multiple); ductal carcinoma in situ; invasive lobular carcinoma; pre-eclampsia (multiple); and urinary system cancer (multiple).

Mouse Erbb2 Target Information

Mouse Erbb2 Predicted Functions

Predicted to enable several functions, including ErbB-3 class receptor binding activity; Hsp90 protein binding activity; and enzyme binding activity. Predicted to contribute to growth factor binding activity. Involved in positive regulation of GTPase activity and positive regulation of protein phosphorylation. Acts upstream of or within several processes, including negative regulation of immature T cell proliferation in thymus; nervous system development; and neuromuscular junction development. Located in apical plasma membrane; cytoplasmic vesicle; and myelin sheath. Is expressed in several structures, including adrenal gland; alimentary system; central nervous system; genitourinary system; and sensory organ. Used to study Hirschsprung’s disease; breast cancer; and dilated cardiomyopathy. Human ortholog(s) of this gene implicated in carcinoma (multiple); high grade glioma; reproductive organ cancer (multiple); and urinary bladder cancer. Orthologous to human ERBB2 (erb-b2 receptor tyrosine kinase 2).

Rat Erbb2 Target Information

  • Target Symbol: Erbb2, erb-b2 receptor tyrosine kinase 2
  • Alias:
    • Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog)
    • C-erbB-2
    • epidermal growth factor receptor-related protein
    • HER2/neu
    • LOC102552659
    • neu
    • NEU proto-oncogene
    • p185erbB2
    • p185neu
    • proto-oncogene c-ErbB-2
    • proto-oncogene NEU
    • receptor tyrosine-protein kinase erbB-2
    • receptor tyrosine-protein kinase erbB-2-like
    • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
    • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2
    • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
    • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • NCBI_Gene: 24337

Rat Erbb2 Predicted Functions

Enables Hsp90 protein binding activity; protein tyrosine kinase activity; and ubiquitin protein ligase binding activity. Involved in several processes, including animal organ development; nervous system development; and positive regulation of intracellular signal transduction. Located in several cellular components, including basolateral plasma membrane; lateral loop; and microvillus. Biomarker of congestive heart failure and median neuropathy. Human ortholog(s) of this gene implicated in carcinoma (multiple); dilated cardiomyopathy; high grade glioma; reproductive organ cancer (multiple); and urinary bladder cancer. Orthologous to human ERBB2 (erb-b2 receptor tyrosine kinase 2).

ERBB2 Target News

    Tumor Marker

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