EK1187

Human Her3/ErbB3 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.11 pg/mL
Array Range93.75 - 6000 pg/mL
Assay Time3.5 h
Recovery82% - 102%
Average Recovery90%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.11 pg/mL
Array Range93.75 - 6000 pg/mL
Assay Time3.5 h
Recovery82% - 102%
Average Recovery90%
Intra Precision2.3% - 5.1%
Inter Precision2.5% - 3.5%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
ComponentsErbB3 Microplate
ErbB3 Standard
ErbB3 Detect Antibody
Standard Diluent
Assay Buffer
Substrate
Stop Solution
Washing Buffer
Plate Covers
Assay PrincipleThis kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-human ErbB3 antibodies are pre-coated on a high-affinity ELISA plate. Standard samples, test samples, and detection antibodies labeled with horseradish peroxidase (HRP) are added to the wells of the ELISA plate. After incubation, the ErbB3 present in the sample binds to the solid-phase antibody and the detection antibody. After washing, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of ErbB3 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

ERBB3

No description available.

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