Human IFN-α ELISA Kit

$245.00 – $350.00

Related Target	IFNA1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL, cell culture supernatant: 100 μL
Sensitivity	1.04 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	97% – 118%
Average Recovery	106%

ELISA Kit Detail Information

Related Target	IFNA1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL, cell culture supernatant: 100 μL
Sensitivity	1.04 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	97% – 118%
Average Recovery	106%
Intra Precision	4.5% – 5.3%
Inter Precision	3.9% – 6.5%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IFN-α monoclonal antibody Human IFN-α freeze-dried standard IFN-α detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IFN-α antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IFN-α present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IFN-α in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IFNA1

No description available.

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