Human IGF-1R/CD221 ELISA Kit

$245.00 – $350.00

Related Target	IGF1R
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	13.27 pg/mL
Array Range	0.22 ng/mL – 14 ng/mL
Assay Time	3.5 h
Recovery	73% – 113%
Average Recovery	91%

ELISA Kit Detail Information

Related Target	IGF1R
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	13.27 pg/mL
Array Range	0.22 ng/mL – 14 ng/mL
Assay Time	3.5 h
Recovery	73% – 113%
Average Recovery	91%
Intra Precision	4.2% – 9.2%
Inter Precision	5.7% - 9.7%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGF-1R/CD221 monoclonal antibody Human IGF-1R/CD221 freeze-dried standard IGF-1R/CD221 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGF-1R antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IGF-1R present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGF-1R in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IGF1R

IGF1R Target Infomation Overview

Target Symbol: IGF1R, insulin like growth factor 1 receptor
Gene Groups: Receptor tyrosine kinases; CD molecules; Fibronectin type III domain containing; MicroRNA protein coding host genes
Alias: JTK13; CD221; IGFIR; MGC18216; IGFR
Alias Names: insulin-like growth factor 1 receptor

IGF1R, insulin like growth factor 1 receptor Target Infomation by Species

Human
Mouse
Rat

Human IGF1R Target Information

Target Symbol: IGF1R, insulin like growth factor 1 receptor
Alias:
- CD221
- IGF-I receptor
- IGFIR
- IGFR
- insulin-like growth factor 1 receptor
- insulin-like growth factor I receptor
- JTK13
- MGC142170
- MGC142172
- MGC18216
- soluble IGF1R variant 1
- soluble IGF1R variant 2
NCBI_Gene: 3480
UniProtKB: P08069

Human IGF1R Predicted Functions

Enables several functions, including insulin binding activity; insulin receptor binding activity; and insulin receptor substrate binding activity. Involved in several processes, including cellular response to amyloid-beta; insulin-like growth factor receptor signaling pathway; and regulation of MAPK cascade. Acts upstream of or within peptidyl-tyrosine autophosphorylation. Located in intracellular membrane-bounded organelle and membrane. Part of alphav-beta3 integrin-IGF-1-IGF1R complex; protein kinase complex; and receptor complex. Implicated in several diseases, including IgA glomerulonephritis; dementia (multiple); kidney cancer (multiple); liver disease (multiple); and nervous system cancer (multiple). Biomarker of several diseases, including Kuhnt-Junius degeneration; autoimmune disease (multiple); endocrine gland cancer (multiple); neurodegenerative disease (multiple); and prostate disease (multiple).

Mouse Igf1r Target Information

Target Symbol: Igf1r, insulin-like growth factor I receptor
Alias:
- A330103N21Rik
- CD221
- hydrops fetalis
- hyft
- IGF-1R
- line 186
- RIKEN cDNA A330103N21 gene
NCBI_Gene: 16001

Mouse Igf1r Predicted Functions

Enables insulin-like growth factor binding activity. Involved in several processes, including adrenal gland development; positive regulation of cold-induced thermogenesis; and positive regulation of meiotic cell cycle. Acts upstream of or within several processes, including gland development; peptidyl-tyrosine autophosphorylation; and regulation of intracellular signal transduction. Located in T-tubule. Is integral component of membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb; and lung. Human ortholog(s) of this gene implicated in several diseases, including dementia (multiple); kidney cancer (multiple); liver disease (multiple); nervous system cancer (multiple); and neurodegenerative disease (multiple). Orthologous to human IGF1R (insulin like growth factor 1 receptor).

Rat Igf1r Target Information

Target Symbol: Igf1r, insulin-like growth factor 1 receptor
Alias:
- IGF-1 receptor
- IGF-I receptor
- IGFIRC
- Igfr1
- insulin-like growth factor I receptor
- JTK13
- type-I IGF receptor
NCBI_Gene: 25718
- UniprotKB: A0A140TAB8
- UniprotKB: P24062

Rat Igf1r Predicted Functions

Enables G-protein alpha-subunit binding activity; insulin receptor substrate binding activity; and insulin-like growth factor-activated receptor activity. Involved in several processes, including cellular response to hormone stimulus; negative regulation of apoptotic process; and nervous system development. Located in several cellular components, including T-tubule; caveola; and neuronal cell body. Used to study Alzheimer’s disease; Parkinson’s disease; and prostate adenocarcinoma. Biomarker of several diseases, including alcoholic neuropathy; artery disease (multiple); cryptorchidism; diabetes mellitus (multiple); and neurodegenerative disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including IgA glomerulonephritis; dementia (multiple); kidney cancer (multiple); liver disease (multiple); and nervous system cancer (multiple). Orthologous to human IGF1R (insulin like growth factor 1 receptor).

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