Human IGFBP-3 ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 2.16 pg/mL |
| Array Range | 125 pg/mL – 8000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 90% – 111% |
| Average Recovery | 102% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 2.16 pg/mL |
| Array Range | 125 pg/mL – 8000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 90% – 111% |
| Average Recovery | 102% |
| Intra Precision | 3.9% – 5.1% |
| Inter Precision | 6.5% – 7.2% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TRAIL/TNFSF10 monoclonal antibody Human TRAIL/TNFSF10 freeze-dried standard TRAIL/TNFSF10 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IGFBP-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IGFBP3
IGFBP3 Target Infomation Overview
- Target Symbol: IGFBP3, insulin like growth factor binding protein 3
- Gene Groups: Insulin like growth factor binding proteins
- Alias: IBP3; BP-53
- Alias Names: growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex; binding protein 53; binding protein 29; IGF-binding protein 3; insulin-like growth factor binding protein 3
IGFBP3, insulin like growth factor binding protein 3 Target Infomation by Species
- Human
- Mouse
- Rat
Human IGFBP3 Target Information
- Target Symbol: IGFBP3, insulin like growth factor binding protein 3
- Alias:
- acid stable subunit of the 140 K IGF complex
- binding protein 29
- binding protein 53
- BP-53
- growth hormone-dependent binding protein
- IBP-3
- IBP3
- IGF-binding protein 3
- IGFBP-3
- insulin-like growth factor binding protein 3
- insulin-like growth factor-binding protein 3
- NCBI_Gene: 3486
- UniProtKB: P17936
Human IGFBP3 Predicted Functions
Enables insulin-like growth factor I binding activity and protein tyrosine phosphatase activator activity. Involved in several processes, including negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; and positive regulation of myoblast differentiation. Acts upstream of or within negative regulation of cell population proliferation; negative regulation of protein phosphorylation; and protein phosphorylation. Located in extracellular space and nucleus. Part of insulin-like growth factor ternary complex. Implicated in several diseases, including Alzheimer’s disease; Turner syndrome; diabetes mellitus (multiple); prostate cancer; and transitional cell carcinoma. Biomarker of several diseases, including Barrett’s esophagus; Down syndrome; autoimmune disease (multiple); bone disease (multiple); and urinary system cancer (multiple).
Mouse Igfbp3 Target Information
- Target Symbol: Igfbp3, insulin-like growth factor binding protein 3
- Alias:
- AI649005
- expressed sequence AI649005
- IGFBP-3
- NCBI_Gene: 16009
Mouse Igfbp3 Predicted Functions
Enables fibronectin binding activity and insulin-like growth factor binding activity. Acts upstream of or within several processes, including osteoblast differentiation; positive regulation of signal transduction; and type B pancreatic cell proliferation. Located in extracellular space. Is expressed in several structures, including alimentary system; brain; early embryo; gonad; and limb. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease; Turner syndrome; diabetes mellitus (multiple); prostate cancer; and transitional cell carcinoma. Orthologous to human IGFBP3 (insulin like growth factor binding protein 3).
Rat Igfbp3 Target Information
- Target Symbol: Igfbp3, insulin-like growth factor binding protein 3
- Alias:
- IBP-3
- IGF-binding protein 3
- IGF-BP3
- IGFBP-3
- Insulin-like growth factor-binding protein (IGF-BP3)
- insulin-like growth factor-binding protein 3
- MGC156773
- NCBI_Gene: 24484
Rat Igfbp3 Predicted Functions
Enables insulin-like growth factor II binding activity. Involved in several processes, including cellular response to Thyroglobulin triiodothyronine; cellular response to estradiol stimulus; and response to peptide hormone. Located in extracellular space; heterochromatin; and platelet alpha granule. Part of insulin-like growth factor binary complex and insulin-like growth factor ternary complex. Used to study impotence. Biomarker of congenital diaphragmatic hernia; impotence; and obesity. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease; Turner syndrome; diabetes mellitus (multiple); prostate cancer; and transitional cell carcinoma. Orthologous to human IGFBP3 (insulin like growth factor binding protein 3).
Products with the Same Target - IGFBP3
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