Human IL-1α ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 50 μL 1×Assay Buffer and 50 μL sample |
| Sensitivity | 0.52 pg/mL |
| Array Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 81% – 106% |
| Average Recovery | 93% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 50 μL 1×Assay Buffer and 50 μL sample |
| Sensitivity | 0.52 pg/mL |
| Array Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 81% – 106% |
| Average Recovery | 93% |
| Intra Precision | 2.5% – 4.4% |
| Inter Precision | 3.9% – 6.8% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1α monoclonal antibody Human IL-1α freeze-dried standard IL-1α detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-1α antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-1α present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1α in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL1A
IL1A Target Infomation Overview
- Target Symbol: IL1A, interleukin 1 alpha
- Gene Groups: Interleukins
- Alias: IL1F1; IL-1A; IL1-ALPHA
- Previous Names: IL1
- Alias Names: preinterleukin 1 alpha; hematopoietin-1; pro-interleukin-1-alpha
IL1A, interleukin 1 alpha Target Infomation by Species
- Human
- Mouse
- Rat
Human IL1A Target Information
- Target Symbol: IL1A, interleukin 1 alpha
- Alias:
- hematopoietin-1
- IL-1 alpha
- IL-1A
- IL1
- IL1-ALPHA
- IL1F1
- interleukin 1, alpha
- interleukin-1 alpha
- preinterleukin 1 alpha
- pro-interleukin-1-alpha
- NCBI_Gene: 3552
- UniProtKB: P01583
Human IL1A Predicted Functions
Enables copper ion binding activity and cytokine activity. Involved in several processes, including positive regulation of interleukin-2 production; positive regulation of mitotic nuclear division; and response to copper ion. Located in cytosol and extracellular space. Implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); cholesteatoma (multiple); common cold; cystic fibrosis; and osteoarthritis.
Mouse Il1a Target Information
- Target Symbol: Il1a, interleukin 1 alpha
- Alias:
- Il-1a
- NCBI_Gene: 16175
Mouse Il1a Predicted Functions
Enables cytokine activity and interleukin-1 receptor binding activity. Involved in positive regulation of angiogenesis and positive regulation of vascular endothelial growth factor production. Acts upstream of or within several processes, including cell surface receptor signaling pathway; connective tissue replacement involved in inflammatory response wound healing; and positive regulation of cytokine production. Located in cytoplasm and extracellular space. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; reproductive system; and respiratory system. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Orthologous to human IL1A (interleukin 1 alpha).
Rat Il1a Target Information
- Target Symbol: Il1a, interleukin 1 alpha
- Alias:
- IL-1 alpha
- IL-1F1
- interleukin-1 alpha
- NCBI_Gene: 24493
Rat Il1a Predicted Functions
Predicted to enable copper ion binding activity; cytokine activity; and interleukin-1 receptor binding activity. Involved in several processes, including keratinization; negative regulation of establishment of Sertoli cell barrier; and positive regulation of intracellular signal transduction. Located in several cellular components, including cell surface; cytosol; and extracellular space. Used to study interstitial lung disease. Biomarker of several diseases, including endometritis; lung disease (multiple); urinary tract infection; uveitis; and varicocele. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Orthologous to human IL1A (interleukin 1 alpha).
Products with the Same Target - IL1A
Citations for Human IL-1α ELISA Kit
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ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
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ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
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