Human IL-16 ELISA Kit

$350.00$450.00

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TARGETSIL15
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.65 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery88% – 112%
Average Recovery1.01
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.65 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery88% – 112%
Average Recovery1.01
Intra Precision2.4% – 4.5%
Inter-Precision3.0% – 6.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-15/IL-15R monoclonal antibody
Mouse IL-15/IL-15R freeze-dried standard
IL-15/IL-15R detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-15/IL-15R antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-15/IL-15R present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-15/IL-15R in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL15

IL15 Target Infomation Overview

  • Target Symbol: IL15, interleukin 15
  • Gene Groups: Interleukins
  • Alias: IL-15; MGC9721

IL15, interleukin 15 Target Infomation by Species

HumanMouseRat

Human IL15 Target Information

Human IL15 Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; macrophage differentiation; and positive regulation of macromolecule metabolic process. Located in cytosol and nuclear speck. Implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Biomarker of several diseases, including artery disease (multiple); brain infarction; lung disease (multiple); obesity; and viral infectious disease (multiple).

Mouse Il15 Target Information

Mouse Il15 Predicted Functions

Enables cytokine activity. Involved in negative regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including T cell activation; lymph node development; and regulation of lymphocyte activation. Located in cytoplasm. Is expressed in several structures, including adrenal gland; genitourinary system; gut; immune system; and nervous system. Human ortholog(s) of this gene implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Orthologous to human IL15 (interleukin 15).

Rat Il15 Target Information

Rat Il15 Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including cellular response to vitamin D; positive regulation of tyrosine phosphorylation of STAT protein; and skeletal muscle atrophy. Located in cell surface and extracellular space. Used to study pancreatitis. Biomarker of listeriosis and periodontal disease. Human ortholog(s) of this gene implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Orthologous to human IL15 (interleukin 15).

IL15 Target News

    4.6
    Transient regulatory-T-cell interruption promotes skin-resident memory T cells mediated tumor protection
    Scientific Reports

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