Human IL-29 ELISA Kit

$245.00 – $350.00

Related Target	IFNL1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	0.12 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	87% – 101%
Average Recovery	93%

ELISA Kit Detail Information

Related Target	IFNL1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	0.12 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	87% – 101%
Average Recovery	93%
Intra Precision	4.6% – 4.7%
Inter Precision	4.2% – 6.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-29 monoclonal antibody Human IL-29 freeze-dried standard IL-29 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-29 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-29 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-29 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IFNL1

IFNL1 Target Infomation Overview

Target Symbol: IFNL1, interferon lambda 1
Gene Groups: Interferons
Alias: IL-29
Previous Names: IL29
Alias Names: interleukin 29; interleukin 29 (interferon, lambda 1)

IFNL1, interferon lambda 1 Target Infomation by Species

Human
Mouse
Rat

Human IFNL1 Target Information

Target Symbol: IFNL1, interferon lambda 1
Alias:
- cytokine Zcyto21
- IFN-lambda-1
- IL-29
- IL29
- interferon lambda-1
- interferon-lambda-1
- interferon, lambda 1
- interleukin 29 (interferon, lambda 1)
- interleukin-29
NCBI_Gene: 282618
UniProtKB: Q8IU54

Human IFNL1 Predicted Functions

Enables interleukin-28 receptor binding activity. Involved in several processes, including negative regulation of immune response; positive regulation of macromolecule metabolic process; and regulation of gene expression. Located in extracellular region. Part of interleukin-28 receptor complex. Implicated in COVID-19; dengue hemorrhagic fever; hepatitis C; and hepatocellular carcinoma. Biomarker of asthma; dengue disease; subacute sclerosing panencephalitis; and tropical spastic paraparesis.

Mouse Ifnl2 Target Information

Target Symbol: Ifnl2, interferon lambda 2
Alias:
- EG330496
- Il28a
- interleukin 28A
- predicted gene, EG330496
NCBI_Gene: 330496
- UniprotKB: Q4VK74
- UniprotKB: B9EK98

Mouse Ifnl2 Predicted Functions

Predicted to enable interleukin-28 receptor binding activity. Involved in defense response to virus and mucosal immune response. Predicted to be located in extracellular region. Predicted to be part of interleukin-28 receptor complex. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in several diseases, including COVID-19; cryoglobulinemia; dengue hemorrhagic fever; hepatitis C; and liver disease (multiple). Orthologous to several human genes including IFNL2 (interferon lambda 2) and IFNL3 (interferon lambda 3).

Rat Ifnl1 Target Information

Target Symbol: Ifnl1, interferon, lambda 1
Alias:
- If1ia2
- interferon 1ia2
- interferon lambda-2-like
- interleukin-28A-like
- LOC100911249
NCBI_Gene: 100911249
UniProtKB: A0A0G2JWM7

Rat Ifnl1 Predicted Functions

Predicted to enable interleukin-28 receptor binding activity. Predicted to be involved in several processes, including defense response to other organism; negative regulation of immune response; and regulation of gene expression. Predicted to be located in extracellular region. Predicted to be part of interleukin-28 receptor complex. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in several diseases, including COVID-19; cryoglobulinemia; dengue hemorrhagic fever; hepatitis C; and liver disease (multiple). Orthologous to human IFNL1 (interferon lambda 1); IFNL2 (interferon lambda 2); and IFNL3 (interferon lambda 3).

Citations for Human IL-29 ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.